Association mapping

Association mapping (genetics), also known as "linkage disequilibrium mapping", is a method of mapping quantitative trait loci (QTLs) that takes advantage of historic linkage disequilibrium to link phenotypes (observable characteristics) to genotypes (the genetic constitution of organisms), uncovering genetic associations.

Theory

Association mapping is based on the idea that traits that have entered a population only recently will still be linked to the surrounding genetic sequence of the original evolutionary ancestor, or in other words, will more often be found within a given haplotype, than outside of it. It is most often performed by scanning the entire genome for significant associations between a panel of SNPs (which, in many cases are spotted onto glass slides to create “SNP chips”) and a particular phenotype. These associations must then be independently verified in order to show that they either (a) contribute to the trait of interest directly, or (b) are linked to/ in linkage disequilibrium with a quantitative trait locus (QTL) that contributes to the trait of interest.[1]

Use

The advantage of association mapping is that it can map quantitative traits with high resolution in a way that is statistically very powerful. Association mapping, however, also requires extensive knowledge of SNPs within the genome of the organism of interest, and is therefore difficult to perform in species that have not been well studied or do not have well-annotated genomes.[2] Association mapping has been most widely applied to the study of human disease, specifically in the form of a genome-wide association study (GWAS). A genome-wide association study is performed by scanning an entire genome for SNPs associated with a particular trait of interest, or in the case of human disease, with a particular disease of interest.[1][3] To date, thousands of genome wide associations studies have been performed on the human genome in attempt to identify SNPs associated with a wide variety of complex human diseases (e.g. cancer, Alzheimer's disease, and obesity). The results of all such published GWAS are maintained in an NIH database (figure 1). Whether or not these studies have been clinically and/or therapeutically useful, however, remains controversial.[3]

Types and Variations

(A) Association mapping in population where members are assumed to be independent.

Several standard methods to test for association. Case control studies – Case control studies was among the first approaches utilized to determine whether particular genetic variant is associated with increased risk of disease in humans. Early on, Woofle in 1955, proposed a relative risk statistic that could be used to assess genotype dependent risk. However persistent concern regarding these studies is the adequacy of matching cases and controls. In particular, population stratification can produce false positive associations. In response to this concern, Falk and Rubenstein in 1987, suggested a method for assessing relative risk that uses family based controls, obviating this source of potential error. Basically, the method uses a control sample of the parental alleles or haplotypes not transmitted to affected offspring.

(B) Association mapping population where members are assumed to be related

In the real world it is very hard to find independent (unrelated) individuals. Population based association mapping has been modified to control population stratification or relatedness in nested association mapping. Still there is one other limitation in population based QTL mapping; when the frequency of the favorable allele should be relatively high to be detected. Usually favorable alleles are rare mutant alleles ( for example usually a resistant parent might be 1 out of 10000 genotypes). Another variant of association mapping in related populations is family based association mapping. In family based association mapping instead of multiple unrelated individuals multiple unrelated families or pedigrees are used. The family based association mapping [4] (external link) can be used in situations where the mutant alleles have been introgressed in populations. One of popular family based association mapping Transmission disequilibrium test. For details please read Family based QTL mapping.

See also

References

  1. 1 2 Gibson, G.; Muse S.V. (2009). A Primer of Genome Science. MA: Sinauer Associates.
  2. ↑ Yu, J.; Holland, J.B.; McMullen, M.D.; Buckler, E.S. (2008). "Genetic design and statistical power of nested association mapping in maize". Genetics. 178 (1): 539–551. doi:10.1534/genetics.107.074245. PMC 2206100Freely accessible. PMID 18202393.
  3. 1 2 Nussbaum, R.L.; McInnes, R.R.; Willard, H.F. (2007). Genetics in Medicine. Philadelphia, PA: Saunders Elsevier.
  4. ↑ Rosyara U.R., J.L. Gonzalez-Hernandez, K.D. Glover, K.R. Gedye and J.M. Stein. 2009. Family-based mapping of quantitative trait loci in plant breeding populations with resistance to Fusarium head blight in wheat as an illustration Theoretical Applied Genetics 118:1617-1631
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