DLGAP5

DLGAP5
Identifiers
Aliases	DLGAP5, DLG7, HURP, discs large homolog associated protein 5, DLG associated protein 5
External IDs	MGI: 2183453 HomoloGene: 8840 GeneCards: DLGAP5
Gene ontology Molecular function • protein binding • phosphoprotein phosphatase activity Cellular component • microtubule organizing center • nucleus • cytoskeleton • spindle • spindle pole centrosome • cytoplasm Biological process • positive regulation of mitotic metaphase/anaphase transition • cell cycle • mitotic chromosome movement towards spindle pole • cell proliferation • signaling • protein dephosphorylation Sources:Amigo / QuickGO
RNA expression pattern
More reference expression data
Orthologs
Species	Human	Mouse
Entrez	9787	218977
Ensembl	ENSG00000126787	ENSMUSG00000037544
UniProt	Q15398	Q8K4R9
RefSeq (mRNA)	NM_001146015 NM_014750	NM_001145949 NM_144553
RefSeq (protein)	NP_001139487.1 NP_055565.3	NP_653136.2
Location (UCSC)	Chr 14: 55.15 – 55.19 Mb	Chr 14: 47.39 – 47.42 Mb
PubMed search	[1]	[2]
Wikidata

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Disks large-associated protein 5 (DAP-5) also known as discs large homolog 7 (DLG7) or hepatoma up-regulated protein (HURP) is a protein that in humans is encoded by the DLGAP5 gene.^[3][4][5][6]

DLG7 is a kinetochore protein that stabilizes microtubules in vicinity of chromosomes. DLG7 controls spindle dynamics, promotes interkinetochore tension and efficient kinetochore capture.^[7] DGL7 is a part of Ran-dependent complex.^[8] Stabilization of DGL7 in cell occurs due to phosphorylation by Aurora A kinase.^[9] Expression of DGL7 is found in cancer^[10] and stem cells.^[11]

References

1. "Human PubMed Reference:".
2. "Mouse PubMed Reference:".
3. Nomura N, Miyajima N, Sazuka T, Tanaka A, Kawarabayasi Y, Sato S, Nagase T, Seki N, Ishikawa K, Tabata S (Dec 1995). "Prediction of the coding sequences of unidentified human genes. I. The coding sequences of 40 new genes (KIAA0001-KIAA0040) deduced by analysis of randomly sampled cDNA clones from human immature myeloid cell line KG-1". DNA Res. 1 (1): 27–35. doi:10.1093/dnares/1.1.27. PMID 7584026.
4. Nomura N, Miyajima N, Sazuka T, Tanaka A, Kawarabayasi Y, Sato S, Nagase T, Seki N, Ishikawa K, Tabata S (Dec 1995). "Prediction of the coding sequences of unidentified human genes. I. The coding sequences of 40 new genes (KIAA0001-KIAA0040) deduced by analysis of randomly sampled cDNA clones from human immature myeloid cell line KG-1 (supplement)". DNA Res. 1 (1): 47–56. doi:10.1093/dnares/1.1.47. PMID 7584028.
5. "Entrez Gene: DLG7 discs, large homolog 7 (Drosophila)".
6. Bassal S, Nomura N, Venter D, Brand K, McKay MJ, van der Spek PJ (September 2001). "Characterization of a novel human cell-cycle-regulated homologue of Drosophila dlg1". Genomics. 77 (1–2): 5–7. doi:10.1006/geno.2001.6570. PMID 11543626.
7. Wilde A (June 2006). ""HURP on" we're off to the kinetochore!". J. Cell Biol. 173 (6): 829–31. doi:10.1083/jcb.200605150. PMC 2063907. PMID 16785318.
8. Koffa MD, Casanova CM, Santarella R, Köcher T, Wilm M, Mattaj IW (April 2006). "HURP is part of a Ran-dependent complex involved in spindle formation". Curr. Biol. 16 (8): 743–54. doi:10.1016/j.cub.2006.03.056. PMID 16631581.
9. Yu CT, Hsu JM, Lee YC, Tsou AP, Chou CK, Huang CY (July 2005). "Phosphorylation and stabilization of HURP by Aurora-A: implication of HURP as a transforming target of Aurora-A". Mol. Cell. Biol. 25 (14): 5789–800. doi:10.1128/MCB.25.14.5789-5800.2005. PMC 1168800. PMID 15987997.
10. Sanderson HS, Clarke PR (June 2006). "Cell biology: Ran, mitosis and the cancer connection". Curr. Biol. 16 (12): R466–8. doi:10.1016/j.cub.2006.05.032. PMID 16782004.
11. Gudmundsson KO, Thorsteinsson L, Sigurjonsson OE, et al. (June 2007). "Gene expression analysis of hematopoietic progenitor cells identifies Dlg7 as a potential stem cell gene". Stem Cells. 25 (6): 1498–506. doi:10.1634/stemcells.2005-0479. PMID 17322106.

Further reading

• Ohara O, Nagase T, Ishikawa K, et al. (1997). "Construction and characterization of human brain cDNA libraries suitable for analysis of cDNA clones encoding relatively large proteins". DNA Res. 4 (1): 53–9. doi:10.1093/dnares/4.1.53. PMID 9179496.
• Bassal S, Nomura N, Venter D, et al. (2001). "Characterization of a novel human cell-cycle-regulated homologue of Drosophila dlg1". Genomics. 77 (1–2): 5–7. doi:10.1006/geno.2001.6570. PMID 11543626.
• Strausberg RL, Feingold EA, Grouse LH, et al. (2003). "Generation and initial analysis of more than 15,000 full-length human and mouse cDNA sequences". Proc. Natl. Acad. Sci. U.S.A. 99 (26): 16899–903. doi:10.1073/pnas.242603899. PMC 139241. PMID 12477932.
• Tsou AP, Yang CW, Huang CY, et al. (2003). "Identification of a novel cell cycle regulated gene, HURP, overexpressed in human hepatocellular carcinoma". Oncogene. 22 (2): 298–307. doi:10.1038/sj.onc.1206129. PMID 12527899.
• Mantovani F, Banks L (2004). "Regulation of the discs large tumor suppressor by a phosphorylation-dependent interaction with the beta-TrCP ubiquitin ligase receptor". J. Biol. Chem. 278 (43): 42477–86. doi:10.1074/jbc.M302799200. PMID 12902344.
• Laprise P, Viel A, Rivard N (2004). "Human homolog of disc-large is required for adherens junction assembly and differentiation of human intestinal epithelial cells". J. Biol. Chem. 279 (11): 10157–66. doi:10.1074/jbc.M309843200. PMID 14699157.
• Shu H, Chen S, Bi Q, et al. (2004). "Identification of phosphoproteins and their phosphorylation sites in the WEHI-231 B lymphoma cell line". Mol. Cell Proteomics. 3 (3): 279–86. doi:10.1074/mcp.D300003-MCP200. PMID 14729942.
• Hsu JM, Lee YC, Yu CT, Huang CY (2004). "Fbx7 functions in the SCF complex regulating Cdk1-cyclin B-phosphorylated hepatoma up-regulated protein (HURP) proteolysis by a proline-rich region". J. Biol. Chem. 279 (31): 32592–602. doi:10.1074/jbc.M404950200. PMID 15145941.
• Blot V, Delamarre L, Perugi F, et al. (2005). "Human Dlg protein binds to the envelope glycoproteins of human T-cell leukemia virus type 1 and regulates envelope mediated cell-cell fusion in T lymphocytes". J. Cell. Sci. 117 (Pt 17): 3983–93. doi:10.1242/jcs.01266. PMID 15286176.
• Beausoleil SA, Jedrychowski M, Schwartz D, et al. (2004). "Large-scale characterization of HeLa cell nuclear phosphoproteins". Proc. Natl. Acad. Sci. U.S.A. 101 (33): 12130–5. doi:10.1073/pnas.0404720101. PMC 514446. PMID 15302935.
• Zhao L, Qin LX, Ye QH, et al. (2005). "KIAA0008 gene is associated with invasive phenotype of human hepatocellular carcinoma--a functional analysis". J. Cancer Res. Clin. Oncol. 130 (12): 719–27. doi:10.1007/s00432-004-0595-2. PMID 15340842.
• Gerhard DS, Wagner L, Feingold EA, et al. (2004). "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)". Genome Res. 14 (10B): 2121–7. doi:10.1101/gr.2596504. PMC 528928. PMID 15489334.
• Yu CT, Hsu JM, Lee YC, et al. (2005). "Phosphorylation and stabilization of HURP by Aurora-A: implication of HURP as a transforming target of Aurora-A". Mol. Cell. Biol. 25 (14): 5789–800. doi:10.1128/MCB.25.14.5789-5800.2005. PMC 1168800. PMID 15987997.
• Rual JF, Venkatesan K, Hao T, et al. (2005). "Towards a proteome-scale map of the human protein-protein interaction network". Nature. 437 (7062): 1173–8. doi:10.1038/nature04209. PMID 16189514.
• Nousiainen M, Silljé HH, Sauer G, et al. (2006). "Phosphoproteome analysis of the human mitotic spindle". Proc. Natl. Acad. Sci. U.S.A. 103 (14): 5391–6. doi:10.1073/pnas.0507066103. PMC 1459365. PMID 16565220.
• Wong J, Fang G (2006). "HURP controls spindle dynamics to promote proper interkinetochore tension and efficient kinetochore capture". J. Cell Biol. 173 (6): 879–91. doi:10.1083/jcb.200511132. PMC 2063914. PMID 16769820.
• Beausoleil SA, Villén J, Gerber SA, et al. (2006). "A probability-based approach for high-throughput protein phosphorylation analysis and site localization". Nat. Biotechnol. 24 (10): 1285–92. doi:10.1038/nbt1240. PMID 16964243.
• Olsen JV, Blagoev B, Gnad F, et al. (2006). "Global, in vivo, and site-specific phosphorylation dynamics in signaling networks". Cell. 127 (3): 635–48. doi:10.1016/j.cell.2006.09.026. PMID 17081983.

External links

• DLG7 protein, human at the US National Library of Medicine Medical Subject Headings (MeSH)