Daisy chaining DNA
Daisy chaining DNA is the process of when DNA undergoing PCR amplification becomes tangled and forms a 'daisy chain.' During PCR, primers or dNTP's will eventually be used up and limit further reactions; it is when the primers deplete that causes daisy chaining. Since the denaturing and annealing processes still continue without primers, the single-stranded DNA molecules reanneal to themselves. However, this reannealing does not always occur to another complementary strand. It is this imperfect match up that causes 'tangles'. These tangles look like a daisy chain.[1]
- ↑ "High-Throughput NGS Library Preparation Technical Guide". Kapa Biosystems. Retrieved 23 September 2015.
|first1=missing|last1=in Authors list (help)
External links
This article is issued from Wikipedia - version of the 9/26/2015. The text is available under the Creative Commons Attribution/Share Alike but additional terms may apply for the media files.