Inducer

In molecular biology, an inducer is a molecule that regulates gene expression.^[1] An inducer can bind to protein repressors or activators.

Inducers function by disabling repressors. The gene is expressed because an inducer binds to the repressor. The binding of the inducer to the repressor prevents the repressor from binding to the operator. RNA polymerase can then begin to transcribe operon genes.

Inducers also function by binding to activators. Activators generally bind poorly to activator DNA sequences unless an inducer is present. Activator binds to an inducer and the complex binds to the activation sequence and activates target gene.^[2] Removing the inducer stops transcription.^[2]

Because a small inducer molecule is required, the increased expression of the target gene is called induction.^[2] The lactose operon is one example of an inducible system.^[2]

Function

Repressor proteins bind to the DNA strand and prevent RNA polymerase from being able to attach to the DNA and synthesize mRNA. Inducers bind to repressors, causing them to change shape and preventing them from binding to DNA. Therefore, they allow transcription, and thus gene expression, to take place.

For a gene to be expressed, its DNA sequence must be copied (in a process known as transcription) to make a smaller, mobile molecule called messenger RNA (mRNA), which carries the instructions for making a protein to the site where the protein is manufactured (in a process known as translation). Many different types of proteins can affect the level of gene expression by promoting or preventing transcription. In prokaryotes (such as bacteria), these proteins often act on a portion of DNA known as the operator at the beginning of the gene. The promoter is where RNA polymerase, the enzyme that copies the genetic sequence and synthesizes the mRNA, attaches to the DNA strand.

Some genes are modulated by activators, which have the opposite effect on gene expression as repressors. Inducers can also bind to activator proteins, allowing them to bind to the operator DNA where they promote RNA transcription.

Ligands that bind to deactivate activator proteins are not, in the technical sense, classified as inducers, since they have the effect of preventing transcription.

Examples

lac operon

The inducer in the lac operon is allolactose.^[2] If lactose is present in the medium, then a small amount of it will be converted to allolactose by a few molecules of β-galactosidase that are present in the cell.^[3] Allolactose binds to the repressor and decreases the repressor's affinity for the operator site.^[3]

However, when lactose and glucose are both available in the system, the lac operon is repressed. This is because glucose actively prevents the induction of lacZYA.^[2]

ara operon

In the ara operon, arabinose is the inducer.

References

1. Baraniak, P. R.; Nelson, D. M.; Leeson, C. E.; Katakam, A. K.; Friz, J. L.; Cress, D. E.; Hong, Y; Guan, J; Wagner, W. R. (2011). "Spatial control of gene expression within a scaffold by localized inducer release". Biomaterials. 32 (11): 3062–71. doi:10.1016/j.biomaterials.2010.12.037. PMC 3178343. PMID 21269687.
2. Slonczewski, Joan, and John Watkins. Foster. Microbiology: An Evolving Science. New York: W.W. Norton &, 2009. Print.
3. Biology Part 2. Berkeley, CA: Berkeley Review, 1995. Print.

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