Isoflavone 7-O-methyltransferase

Isoflavone 7-O-methyltransferase
Identifiers
EC number	2.1.1.150
CAS number	136111-54-1
Databases
IntEnz	IntEnz view
BRENDA	BRENDA entry
ExPASy	NiceZyme view
KEGG	KEGG entry
MetaCyc	metabolic pathway
PRIAM	profile
PDB structures	RCSB PDB PDBe PDBsum
Search PMC articles PubMed articles NCBI proteins

In enzymology, an isoflavone 7-O-methyltransferase (EC 2.1.1.150) is an enzyme that catalyzes the chemical reaction

S-adenosyl-L-methionine + a 7-hydroxyisoflavone $\rightleftharpoons$ S-adenosyl-L-homocysteine + a 7-methoxyisoflavone

Thus, the two substrates of this enzyme are S-adenosyl methionine and 7-hydroxyisoflavone, whereas its two products are S-adenosylhomocysteine and 7-methoxyisoflavone.

This enzyme belongs to the family of transferases, specifically those transferring one-carbon group methyltransferases. The systematic name of this enzyme class is S-adenosyl-L-methionine:hydroxyisoflavone 7-O-methyltransferase. This enzyme participates in isoflavonoid biosynthesis.

References

• Edwards R, Dixon RA (1991). "Isoflavone O-methyltransferase activities in elicitor-treated cell suspension cultures of Medicago sativa". Phytochemistry. 30 (8): 2597–2606. doi:10.1016/0031-9422(91)85107-B.
• He XZ, Dixon RA (2000). "Genetic manipulation of isoflavone 7-O-methyltransferase enhances biosynthesis of 4'-O-methylated isoflavonoid phytoalexins and disease resistance in alfalfa". Plant. Cell. 12 (9): 1689–702. doi:10.1105/tpc.12.9.1689. PMC 149079. PMID 11006341.
• He XZ, Dixon RA (1996). "Affinity chromatography, substrate/product specificity, and amino acid sequence analysis of an isoflavone O-methyltransferase from alfalfa (Medicago sativa L.)". Arch. Biochem. Biophys. 336 (1): 121–9. doi:10.1006/abbi.1996.0539. PMID 8951042.
• He XZ, Reddy JT, Dixon RA (1998). "Stress responses in alfalfa (Medicago sativa L). XXII. cDNA cloning and characterization of an elicitor-inducible isoflavone 7-O-methyltransferase". Plant. Mol. Biol. 36 (1): 43–54. doi:10.1023/A:1005938121453. PMID 9484461.
• Liu CJ, Dixon RA (2001). "Elicitor-induced association of isoflavone O-methyltransferase with endomembranes prevents the formation and 7-O-methylation of daidzein during isoflavonoid phytoalexin biosynthesis". Plant. Cell. 13 (12): 2643–58. doi:10.1105/tpc.13.12.2643. PMC 139479. PMID 11752378.
• Zubieta C, He XZ, Dixon RA, Noel JP (2001). "Structures of two natural product methyltransferases reveal the basis for substrate specificity in plant O-methyltransferases". Nat. Struct. Biol. 8 (3): 271–9. doi:10.1038/85029. PMID 11224575.
• Christensen AB, Gregersen PL, Olsen CE, Collinge DB (1998). "A flavonoid 7-O-methyltransferase is expressed in barley leaves in response to pathogen attack". Plant. Mol. Biol. 36 (2): 219–27. doi:10.1023/A:1005985609313. PMID 9484434.