Lalji Singh

Lalji Singh

Photograph of Dr Lalji Singh and Indian Scientist

Lalji Singh in 1992 with Indian Rock Python
Born (1947-07-09) 9 July 1947
Kalwari, United Provinces, British India
Residence Hyderabad
Nationality Indian
Fields
Institutions
Alma mater Banaras Hindu University
Doctoral advisor S. P. Ray-Chaudhuri
Known for
Notable awards
Spouse Amarawati Singh[1]
Children
  • Abhishek Singh
  • Praveen Kumar Singh

[1]

Lalji Singh (born 5 July 1947) is an Indian scientist who has worked in the field of DNA fingerprinting technology in India, where he is popularly known as the "Father of Indian DNA fingerprinting". Singh has also worked in the areas of molecular basis of sex determination, Wildlife conservation forensics and evolution and migration of humans. In 2004, he received the Padma Shri in recognition of his contribution to Indian science and technology.[2]

Singh founded various institutes and laboratories in India, including the Centre for DNA Fingerprinting and Diagnostics in 1995, Laboratory for the Conservation of Endangered Species (LaCONES) in 1998, and Genome Foundation in 2004, aiming to diagnose and treat genetic disorders affecting the Indian population, in particular the under-privileged people residing in rural India.

Singh also served as the 25th Vice Chancellor of Banaras Hindu University (BHU) and Chairman of Board of Governors of Indian Institute of Technology (BHU) Varanasi from August 2011 to August 2014. Before his term as Vice Chancellor of Banaras Hindu University, he also served as director of the Centre for Cellular and Molecular Biology (CCMB) from May 1998 to July 2009 and Officer on Special Duty (OSD) of Centre for DNA Fingerprinting and Diagnostics (CDFD), Hyderabad, India in 1995–1999.

Education and research career

Early life and education

Lalji Singh was born and raised in a small village Kalwari in Jaunpur District of Uttar Pradesh, India. His father, Suryanarayan Singh, was a farmer and used to serve as head of the village. Singh underwent his primary education up to eighth standard at a government school in Kalwari. However, as there were no further education facilities in the village for senior classes, he was admitted at another school in the nearby village of Pratapganj 6–7 km (3.7–4.3 mi) from his village. After completing his 12th standard in the science group from this school, he attended Banaras Hindu University to pursue his graduation in Zoology and cytogenetics.[3]

University education

Lalji Singh (Second from right) in 1968 at BHU, Varanasi, India

Singh obtained his B.Sc. degree in 1964 from the Banaras Hindu University (BHU). Singh acquired the highest percentage of marks in his Master's degree at BHU in 1966, and won the Banaras Hindu Gold Medal for standing first in order of merit. He was awarded the Junior Research Fellowship (JRF) from the University Grants Commission (India) in 1966. Singh then went on to receive his doctorate from the Banaras Hindu University in 1971, for his work on "Evolution of karyotypes in snakes" in the area of cytogenetics under the guidance of professor S.P. Ray Chaudhuri.[4] His doctoral thesis was published in Chromosoma, an International peer reviewed journal of Springer Link.[5] Singh received the INSA Medal for Young Scientists in 1974, for his research work in the field of cytogenetics.[4]

Research career

In 1971–72, Singh worked as a research associate at the Department of Zoology, University of Calcutta and in April 1974, he was appointed as pool officer of CSIR. In 1974, Singh received the Commonwealth Fellowship to carry out research at the University of Edinburgh, UK, where he worked until 1987.[6] During that time, Singh also worked as a guest scientist at the University of Calcutta, India, for a short period of time and visited the Australian National University in Canberra as a visiting fellow.[7]

In June 1987, Singh returned to India and joined the Centre for Cellular and Molecular Biology (CCMB) in Hyderabad, as a senior scientist. After coming back to India, Singh developed and established the DNA fingerprinting technology for forensic investigation of crime and civil disputes.[6] In July 1998, Singh became the fourth director of the CCMB, where he served until July 2009.[8] During April 2006 to July 2015, Singh was awarded the J.C. Bose National Research Fellowship of India. He was also awarded the Shanti Swarup Bhatnagar Fellowship of CSIR in January 2010 to December 2014.[3] On 22 August 2009, Singh was appointed as the 25th Vice Chancellor of Banaras Hindu University in Varanasi, India.[9][10][11][12] During his three-year tenure as vice chancellor at BHU, Singh took ₹ 1 as a token salary from the university.[13][14]

As of August 2014, Singh is involved in several other academic and research organisations in various capacities. These include being a member of the Governing Board of Indian Council of Agricultural Research (ICAR), New Delhi; chairman of the Research Advisory Council (RAC) of National Bureau of Animal Genetic Resources (NBAGR), Karnal; chairman of the RAC of Project Directorate on Poultry (PDP), Hyderabad; member of the advisory committee of Pharmacopeia Commission of India; chairman of the RAC of National Bureau of Fish Genetic Resources (NBFGR), Lucknow; member of the Board of Management of Sri Ramachandra University, Chennai and member of the Board of Governors of Indian Institutes of Science Education and Research (IISER) Mohali.[7] Singh is also serving as director of the Genome Foundation, a non-profit organization aiming to develop cheap molecular diagnostics for genetic disorders prevalent in rural India.[15]

Singh has published more than 230 research papers during his 45 years of research, and as of April 2013 he has an h-index of 30.[16]

Innovations and contributions to science and technology

DNA fingerprinting technology

During his early science career as a Masters student in 1968, Singh became interested in studying the cytogenetics of Indian snakes.[17] During the 1970s, while studying the evolution of sex chromosome in a species of an Indian snake, the banded krait, Singh and his colleagues identified a highly conserved repeated DNA sequences in the banded krait and other vertebrates,[18][19] which they named the "Banded Krait Minor" (Bkm) sequences in 1980.[20] These Bkm sequences were conserved across various species and were found to be polymorphic in humans.[21] In 1987 through 1988, while working in the CCMB, Singh established that this Bkm-derived probe could be used to generate individual specific DNA Fingerprints of humans for forensic investigations; and in 1988, he used that probe for the first time to solve a case of parentage dispute in India.[22] In 1991, Singh produced the first DNA fingerprinting based evidence in an Indian Court to settle a disputed paternity.[23][24] This was followed by DNA fingerprinting based resolution of hundreds of civil and criminal cases, including cases such as the assassination case(s) of Beant Singh and Rajiv Gandhi,[25] Naina Sahni Tandoor murder case,[26] Swami Premananda case,[25][27] Swami Shraddhananda case,[28] and Priyadarshini Mattoo murder case.[29]

This established the DNA fingerprinting to be used as evidence in the legal system of India.[30] Singh's work in this arena prompted the government of India's Department of Biotechnology to form an autonomous institution, the Centre for DNA Fingerprinting and Diagnostics (CDFD) in 1995, with the aim to provide DNA fingerprinting services to the country, especially for human identification purposes.[7][31]

Singh's lifetime contributions for the development and establishment of indigenous DNA Fingerprinting Technology in India were recognized by the nation, and he is now popularly known as the "Father of DNA fingerprinting" in India.[32][33][34]

Wildlife conservation and forensics

A spotted deer fawn born out of first successful artificial insemination in India at LaCONES on 14 March 2006

While serving as director of CCMB during the end of 20th century, Singh realized an urgent need for assessment and conservation of the wildlife resources of India. To address that need, he conceptualized the Laboratory for conservation of endangered species (LaCONES) in 1998.[35] The foundation stone of this laboratory was laid by the Minister of Environment and Forests, Government of India on 16 September 2001. On 2 February 2007, the laboratory was inaugurated and dedicated to the nation by then President of India A.P.J. Abdul Kalam.[36][37]

The LaCONES is currently serving as the prime research laboratory of India for conservation and preservation of wildlife resources using advanced technological interventions;[38] and also the "National Wildlife Forensic Cell" to provide wildlife identification services for the nation, based on the fundamental discovery of universal primer technology made by Sunil Kumar Verma and Singh (2001).[39][40][41] The first DNA-based test for establishing the species identity of an unknown biological sample pertaining to a case of wildlife crime, was conducted in that laboratory in 2000.[42] Since then, the laboratory has undertaken thousands of such cases on a routine basis for the entire country and has revitalized the field of wildlife forensics.[43]

The laboratory recorded a major success in the Assisted reproductive technology (ART) of wild animals, when the world's first successful artificial insemination of an in estrus induced spotted deer, by non-surgical intra-vaginal insemination done in the laboratory, led to the birth of a live fawn on 14 March 2006.[44][45] This achievement was further followed by an artificial insemination (AI) assisted birth of an Indian blackbuck fawn in August 2007. These were the first reports in the world of such successful AI of wild animals.[46]

On the World Heritage Day in 2013, the LaCONES campus was recognized as a "Heritage Monument" by the Indian National Trust for Art and Culture (Intach)[47] for its notable architecture and design.[48][49]

Molecular basis of sex determination

In 1982, Singh and his colleagues discovered that a tiny portion of the short arm of sex-determining Y chromosome was necessary and sufficient to convert a female mouse to male.[50] This fundamental discovery provided a new concept of Chromosomal translocation of sex-determining region from Y chromosome to X chromosomes causing sex reversal in mice. These findings later became the foundation for the discovery of a similar phenomenon of sex reversal in humans.[51]

DNA-based molecular diagnostics

Until 1998, India did not have an adequate facility for diagnosis of genetic disorders, prevalent in the country. The fundamental research carried out by Singh and his colleagues in that area, led to conceptualization and establishment of the first DNA based diagnostic laboratory in the country.[52] The lab later evolved into a separate institute, the Centre for DNA Fingerprinting and Diagnostics in Hyderabad, India to provide advanced DNA based molecular diagnostics services for the nation.[53]

Novel insights into evolution and migration of humans

The fundamental DNA-based research carried out by Singh and his colleagues on primitive tribes including the tribal population of the Andaman and Nicobar Islands, provided critical insights into the evolution and migration of humans, suggesting the out of Africa route of modern humans about 60,000 years ago to the Andaman Islands.[54] These findings have furthered the scientific community's understanding of the origin of man in terms of evolution and migration from place to place.[55][56]

Genome Foundation

In 2004, Singh founded a non-profit research and service organization, the Genome Foundation, with the aim of diagnosing and treating genetic disorder affecting the Indian population, in particular the under-privileged people residing in rural India, with the participation and voluntary services of scientists and professionals.[15][57]

Books by Singh

Awards and honors

as of October 2014, Singh holds fellowships from several Indian and Foreign academies. These include:

Some of the notable awards conferred to Singh are as follows:

Singh has also been awarded "Honorary D.Sc. degree" by six Universities including the Banaras Hindu University.[7]

Selected publications

References

  1. 1 2 Margadarsi – Indian molecular biologist Lalji Singh (in Telugu). ETV News. 30 May 2013. Retrieved 16 January 2015 – via YouTube.
  2. ↑ "Padma Awards" (PDF). Ministry of Home Affairs, Government of India. 2015. Retrieved July 21, 2015.
  3. 1 2 Vaidya, Manasi T.; Singh, Nandita (9 December 2011). "The Game Changer Geneticist". BioSpectrum. Retrieved 16 December 2014.
  4. 1 2 3 "Lalji Singh: Fellow of Indian National Science Academy". Indian National Science Academy. Retrieved 16 December 2014.
  5. ↑ Singh, Lalji (1972). "Evolution of karyotypes in snakes". Chromosoma. 38 (2): 185–236. doi:10.1007/BF00326193. Retrieved 15 January 2015.
  6. 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 "Professor (Dr) Lalji Singh – A Profile". Centre for Cellular and Molecular Biology. Retrieved 16 December 2014.
  7. 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 "Dr. Lalji Singh: Brief Profile". Banaras Hindu University. Retrieved 16 December 2014.
  8. ↑ "Past Directors of CCMB". CCMB. Retrieved 16 December 2014.
  9. ↑ Rajeev, M. (19 August 2011). "Lalji Singh appointed as BHU Vice-Chancellor". The Hindu. Retrieved 16 December 2014.
  10. ↑ "Padma Shri Lalji Singh is new BHU V-C". The Indian Express. 22 August 2011. Retrieved 16 December 2014.
  11. ↑ PTI (23 August 2014). "BHU's Vice Chancellor Lalji Singh retires". www.indiatvnews.com. Independent News Service. Retrieved 16 December 2014.
  12. ↑ "Tripathi takes charge as BHU V-C". The Indian Express. 28 November 2014. Retrieved 16 December 2014.
  13. ↑ "Dr Lalji Singh to take only Re 1 as wage for BHU VC". WebIndia123. 4 August 2011. Retrieved 16 December 2014.
  14. ↑ "Dr Lalji Singh to take only Re 1 as wage for Banaras Hindu University – Vice Chancellor". IIT-BHU Chronicle. 21 August 2011. Retrieved 15 January 2015.
  15. 1 2 TNN (8 June 2011). "Genome Foundation to set up centres in rural areas". The Times of India. Retrieved 17 December 2014.
  16. ↑ Mukherjee, Bhaskar (September 2013). "A scientometric profile of Prof. Lalji Singh as seen through Web of Science and Scopus" (PDF). Annals of Library and Information Studies. 60: 195–203. Retrieved 16 December 2014.
  17. ↑ Singh, Binay (26 July 2012). "Charm of snakes led this scientist to DNA fingerprinting". The Times of India. Retrieved 16 December 2014.
  18. ↑ Singh, L.; Sharma, T.; Ray-Chaudhuri, S.P. (1970). "Multiple sex chromosomes in the common krait, Bungarus caeruleus Schneider". Chromosoma. 31: 386–391. doi:10.1007/bf00285830. PMID 5490305.
  19. ↑ Singh, L.; Purdom, I.F.; Jones, K.W. (1976). "The chromosomal localization of satellite DNA in Ptyas mucosus (Ophidia, Colubridae)". Chromosoma. 57: 177–184. doi:10.1007/BF00292916.
  20. ↑ Singh, L.; Purdom, I.F.; Jones, K.W. (1980). "Sex chromosome associated satellite DNA: Evolution and conservation". Chromosoma. 79: 137–157. doi:10.1007/bf01175181. PMID 7398495.
  21. ↑ Singh, L.; Jones, K.W. (1986). "Bkm sequences are polymorphic in humans and are clustered in pericentric regions of various acrocentric chromosomes including the Y". Human Genetics. 73: 304–308. doi:10.1007/bf00279091. PMID 3017839.
  22. ↑ Jayaraman, K.S. (1989). "Cut-price fingerprints.". Nature. 340: 175. doi:10.1038/340175c0. PMID 2755472.
  23. ↑ "Kunhiraman vs Manoj on 27 February, 1991 [II (1991) DMC 499]". www.indiankanoon.org. Kerala High Court. Retrieved 17 December 2014.
  24. ↑ Singh, Lalji (1991). "DNA profiling and its application". Current Science. 60: 580–585.
  25. 1 2 "Chandradevi (A-3) vs State Of Tamil Nadu on 12 December, 2002". www.indiankanoon.org. Madras High Court. Retrieved 16 December 2014.
  26. ↑ TNN (21 August 2001). "Nailing a criminal the DNA way". The Times of India. Retrieved 16 December 2014.
  27. ↑ "TWO ASPECTS OF SWAMI PREMANANDA: "THE AVATAR OF THIS AGE" plus CONVICTED RAPIST & MURDERER". www.exbaba.com. Retrieved 16 December 2014.
  28. ↑ "Criminal Appeal No. 454 of 2006, Swamy Shraddananda @ Murali Manohar Mishra vs State Of Karnataka, the Supreme Court of India, decided on 22 July 2008". www.indiankanoon.org. Supreme Court of India. Retrieved 24 January 2015.
  29. ↑ "Santosh Kumar Singh vs State Th. Cbi on 6 October, 2010". www.indiankanoon.org. Supreme Court of India. Retrieved 16 December 2014.
  30. ↑ Rajagopal, Krishnadas (7 October 2010). "SC pulls up trial court for not taking DNA experts seriously". The Indian Express. Retrieved 16 December 2014.
  31. ↑ "Official website of Centre for DNA Fingerprinting and Diagnostics (CDFD)". Retrieved 17 December 2014.
  32. ↑ TNN (4 July 2014). "BHU introduces PG course in forensic science from this session". The Times of India. Retrieved 16 December 2014.
  33. ↑ "DNA inventor Dr Lalji Singh gets Nayudamma Award". Telugu Mirchi. 3 February 2013. Retrieved 16 December 2014.
  34. ↑ TNN (11 November 2012). "Forensic top expert visiting Allahabad for lecture". The Times of India. Retrieved 22 January 2015.
  35. ↑ "President to open LaCONES". Oneindia.com. 20 November 2006. Retrieved 17 December 2014.
  36. ↑ "PRESIDENT 'S ADDRESS AT THE DEDICATION OF THE LABORATORY FOR THE CONSERVATION OF ENDANGERED SPECIES (LACONES), HYDERABAD". Press Information Bureau, Government of India. 1 February 2007. Retrieved 17 December 2014.
  37. ↑ "President dedicates LaCONES to nation". The Hindu: Business Line. 3 February 2007. Retrieved 17 December 2014.
  38. ↑ "Virtual Tour of LaCONES". CSIR-Centre for Cellular and Molecular Biology. Retrieved 19 December 2014.
  39. ↑ Verma, Sunil Kumar; Singh, Lalji. "US Patent No 7141364: Verma and Singh 2001". United States Patent and Trademark Office. Retrieved 29 December 2014.
  40. ↑ Singh, Lalit T. (10 February 2002). "It's only a leopard: lab finding". The Hindu. Retrieved 29 December 2014.
  41. ↑ "CCMB develops DNA test to identify animal species". The Hindu: Business Line. 30 April 2003. Retrieved 18 November 2014.
  42. ↑ "Verma and Singh, Universal Primer technology US and PCT Patent application No: PCT/IN01/00055, First Filling Date: 28/03/2001; Priority date: 28/03/2001". World Intellectual Property Organization. 3 October 2002. Retrieved 19 December 2014.
  43. ↑ "CSIR-Centre for Cellular & Molecular Biology (CSIR-CCMB) had developed DNA barcoding technology". Press Information Bureau, Ministry of Science & Technology, Government of India. 6 August 2014. Retrieved 18 November 2014.
  44. ↑ "CCMB's non-invasive artificial insemination bears fawn". Business Standard. 29 March 2006. Retrieved 17 December 2014.
  45. ↑ TNN (29 March 2006). "CCMB scientists spawn spotted fawn". The Times of India. Retrieved 17 December 2014.
  46. ↑ "LaCONES delivers "BLACKY", a Black Buck" (PDF). Council of Scientific and Industrial Research (CSIR). 24 August 2007. Retrieved 19 December 2014.
  47. ↑ "Hyderabad monuments awarded". Deccan Chronicle. 19 April 2013. Retrieved 17 December 2014.
  48. ↑ Corradi, Mara (29 January 2014). "Shirish Beri: Laboratory for the Conservation of Endangered Species". Floornature. Retrieved 17 December 2014.
  49. ↑ Beri, Shirish; and Associates. "LaCONES (Laboratory for the Conservation of Endangered Species)". Architecture-page. Retrieved 17 December 2014.
  50. ↑ Singh, L.; Jones, K.W. (1982). "Sex reversal in the mouse (Mus musculus) is caused by a recurrent nonreciprocal crossover involving the X and an aberrant Y chromosome" (PDF). Cell. 28: 205–216. doi:10.1016/0092-8674(82)90338-5.
  51. ↑ Guellaen, Georges; Casanova, Myriam; Bishop, Colin; Geldwerth, Danielle; Andre, Gabriel; Fellous, Marc; Weissenbach, Jean (1984). "Human XX males with Y single-copy DNA fragments". Nature. 307: 172–173. doi:10.1038/307172a0.
  52. ↑ "Genetic fingerprinting catches on". Centre for Science and Environment. 30 October 1992. Retrieved 17 December 2014.
  53. ↑ Somasekhar, M. (9 August 2012). "DNA fingerprinting comes of age". The Hindu: Business Line. Retrieved 17 December 2014.
  54. ↑ "DNA polymorphism provides insight into human origin". The Hindu. 4 March 2011. Retrieved 16 December 2014.
  55. ↑ TNN (25 September 2009). "Aryan-Dravidian divide a myth: Study". The Times of India. Retrieved 16 December 2014.
  56. ↑ Chellappan, Kumar (10 December 2011). "New research debunks Aryan invasion theory". Daily News and Analysis. Retrieved 16 December 2014.
  57. ↑ Singh, Lalit T. (13 December 2014). "Even a rupee can help fund its cause". The Hindu. Retrieved 17 December 2014.
  58. ↑ "Lalji Singh: Fellow of TWAS". www.twas.org. The World Academy of Sciences. Retrieved 17 December 2014.
  59. ↑ "CSIR award for CCMB". The Hindu. 24 September 2008. Retrieved 18 November 2014.
  60. ↑ "CCMB bags CSIR technology award for life sciences 2008". WebIndia123. 23 September 2008. Retrieved 18 November 2014.
  61. ↑ "CSIR Technology Awards – 2008". Department of Science and Technology, Government of India. 26 September 2008. Retrieved 18 November 2014.
  62. ↑ "NRDC Awards 2009". National Research Development Corporation. Retrieved 31 December 2014.
  63. ↑ Kelath, Uma (9 January 2012). "BioSpectrum awards excellence". Biospectrum. Retrieved 17 December 2014.
  64. ↑ "Padma Shri Awardees – 2004". Government of India. Retrieved 16 December 2014.

External links

Academic offices
Preceded by
Dr. Balasubramanian		 Director of Centre for Cellular and Molecular Biology
May 1998 – July 2009 		Succeeded by
Dr. Ch. Mohan Rao
Preceded by
D. P. Singh		 Vice Chancellor of Banaras Hindu University
August 2011 – August 2014 		Succeeded by
Prof. G. S. Tripathi
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