MAFA (gene)

MAFA

Transcription factor MafA is a protein that in humans is encoded by the MAFA gene.^[1] It is a member of the Maf family of transcription factors. ^[2]

MAFA Assists in Insulin Regulation

An in vivo study on mice proved MafA binds to the promoter in an insulin gene to regulate insulin transcription in response to serum glucose levels.^[3] MafA is a β cell-specific activator, which differentiates it from other transcription factors involved with insulin gene expression.^[4] It helps regulate the β cells involved with insulin secretion primarily by maintaining β cell metabolism.^[5] The amount of MafA in the β cells is regulated by levels of glucose and oxidative stress.^[2]

MAFA
Available structures PDB Ortholog search: PDBe RCSB List of PDB id codes 4EOT
Identifiers
Aliases	MAFA, RIPE3b1, hMafA, MAF bZIP transcription factor A
External IDs	MGI: 2673307 HomoloGene: 65867 GeneCards: MAFA
Gene ontology Molecular function • transcription factor activity, sequence-specific DNA binding • protein heterodimerization activity • DNA binding • transcriptional activator activity, RNA polymerase II core promoter proximal region sequence-specific binding • RNA polymerase II core promoter proximal region sequence-specific DNA binding • protein homodimerization activity • sequence-specific DNA binding • transcriptional activator activity, RNA polymerase II transcription regulatory region sequence-specific binding Cellular component • nucleus Biological process • regulation of transcription, DNA-templated • response to glucose • insulin secretion • transcription from RNA polymerase II promoter • positive regulation of transcription from RNA polymerase II promoter • regulation of transcription from RNA polymerase II promoter • positive regulation of transcription, DNA-templated • nitric oxide mediated signal transduction • transcription, DNA-templated Sources:Amigo / QuickGO
RNA expression pattern
More reference expression data
Orthologs
Species	Human	Mouse
Entrez	389692	378435
Ensembl	ENSG00000182759	ENSMUSG00000047591
UniProt	Q8NHW3	Q8CF90
RefSeq (mRNA)	NM_201589	NM_194350
RefSeq (protein)	NP_963883.2	NP_919331.1
Location (UCSC)	Chr 8: 143.42 – 143.43 Mb	Chr 15: 75.75 – 75.75 Mb
PubMed search	[6]	[7]
Wikidata

View/Edit Human

View/Edit Mouse

Interactions

MafA (gene) has been shown to interact with NEUROD1^[8] and Pdx1.^[8] MafA works with Pdx1 to activate the insulin gene. ^[2]

References

1. "Entrez Gene: MAFA v-maf musculoaponeurotic fibrosarcoma oncogene homolog A (avian)".
2. Aramata; Han. "Roles and Regulation of Transcription Factor MafA in Islet β-cells". Endocr. J. 54.
3. Zhang; Moriguchi. "MafA Is a Key Regulator of Glucose-Stimulated Insulin Secretion". Mol. Cell. Biol. 25.
4. Matsuoka; Artner. "The MafA transcription factor appears to be responsible for tissue-specific expression of insulin". Proc. Natl. Acad. Sci. U.S.A. 101.
5. Wang; Kataoka. "MAFA controls genes implicated in insulin biosynthesis and secretion.". Diabetologia. 50.
6. "Human PubMed Reference:".
7. "Mouse PubMed Reference:".
8. Zhao, Li; Guo Min; Matsuoka Taka-Aki; Hagman Derek K; Parazzoli Susan D; Poitout Vincent; Stein Roland (Mar 2005). "The islet beta cell-enriched MafA activator is a key regulator of insulin gene transcription". J. Biol. Chem. United States. 280 (12): 11887–94. doi:10.1074/jbc.M409475200. ISSN 0021-9258. PMID 15665000.

Further reading

• Benkhelifa S, Provot S, Nabais E, et al. (2001). "Phosphorylation of MafA is essential for its transcriptional and biological properties.". Mol. Cell. Biol. 21 (14): 4441–52. doi:10.1128/MCB.21.14.4441-4452.2001. PMC 87104. PMID 11416124.
• Olbrot M, Rud J, Moss LG, Sharma A (2002). "Identification of beta-cell-specific insulin gene transcription factor RIPE3b1 as mammalian MafA.". Proc. Natl. Acad. Sci. U.S.A. 99 (10): 6737–42. doi:10.1073/pnas.102168499. PMC 124472. PMID 12011435.
• Kataoka K, Han SI, Shioda S, et al. (2003). "MafA is a glucose-regulated and pancreatic beta-cell-specific transcriptional activator for the insulin gene.". J. Biol. Chem. 277 (51): 49903–10. doi:10.1074/jbc.M206796200. PMID 12368292.
• Matsuoka TA, Artner I, Henderson E, et al. (2004). "The MafA transcription factor appears to be responsible for tissue-specific expression of insulin.". Proc. Natl. Acad. Sci. U.S.A. 101 (9): 2930–3. doi:10.1073/pnas.0306233101. PMC 365722. PMID 14973194.
• Kaneto H, Matsuoka TA, Nakatani Y, et al. (2005). "A crucial role of MafA as a novel therapeutic target for diabetes.". J. Biol. Chem. 280 (15): 15047–52. doi:10.1074/jbc.M412013200. PMID 15664997.
• Harmon JS, Stein R, Robertson RP (2005). "Oxidative stress-mediated, post-translational loss of MafA protein as a contributing mechanism to loss of insulin gene expression in glucotoxic beta cells.". J. Biol. Chem. 280 (12): 11107–13. doi:10.1074/jbc.M410345200. PMID 15664999.
• Zhao L, Guo M, Matsuoka TA, et al. (2005). "The islet beta cell-enriched MafA activator is a key regulator of insulin gene transcription.". J. Biol. Chem. 280 (12): 11887–94. doi:10.1074/jbc.M409475200. PMID 15665000.
• Aramata S, Han SI, Yasuda K, Kataoka K (2005). "Synergistic activation of the insulin gene promoter by the beta-cell enriched transcription factors MafA, Beta2, and Pdx1.". Biochim. Biophys. Acta. 1730 (1): 41–6. doi:10.1016/j.bbaexp.2005.05.009. PMID 15993959.
• Raum JC, Gerrish K, Artner I, et al. (2006). "FoxA2, Nkx2.2, and PDX-1 regulate islet beta-cell-specific mafA expression through conserved sequences located between base pairs -8118 and -7750 upstream from the transcription start site.". Mol. Cell. Biol. 26 (15): 5735–43. doi:10.1128/MCB.00249-06. PMC 1592775. PMID 16847327.