ORC6

ORC6
Available structures PDB Ortholog search: PDBe RCSB List of PDB id codes 3M03
Identifiers
Aliases	ORC6, ORC6L, origin recognition complex subunit 6
External IDs	MGI: 1929285 HomoloGene: 8635 GeneCards: ORC6
Gene ontology Molecular function • DNA binding • protein binding Cellular component • nucleus • membrane • aggresome • nucleoplasm • actin cytoskeleton • origin recognition complex • nuclear origin of replication recognition complex Biological process • G1/S transition of mitotic cell cycle • DNA replication Sources:Amigo / QuickGO
RNA expression pattern
More reference expression data
Orthologs
Species	Human	Mouse
Entrez	23594	56452
Ensembl	ENSG00000091651	ENSMUSG00000031697
UniProt	Q9Y5N6	Q9WUJ8
RefSeq (mRNA)	NM_014321	NM_001163791 NM_019716
RefSeq (protein)	NP_055136.1	n/a
Location (UCSC)	Chr 16: 46.69 – 46.7 Mb	Chr 8: 85.3 – 85.31 Mb
PubMed search	[1]	[2]
Wikidata

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Origin recognition complex subunit 6 (ORC6)
Identifiers
Symbol	ORC6
Pfam	PF05460
InterPro	IPR008721
Available protein structures: Pfam structures PDB RCSB PDB; PDBe; PDBj PDBsum structure summary

Origin recognition complex subunit 6 is a protein that in humans is encoded by the ORC6 (ORC6L) gene.^[3][4]

Background

The origin recognition complex (ORC) is a highly conserved six subunit protein complex essential for the initiation of the DNA replication in eukaryotic cells. Studies in yeast demonstrated that ORC binds specifically to origins of replication and serves as a platform for the assembly of additional initiation factors such as Cdc6 and Mcm proteins.

Function

The protein encoded by this gene is a subunit of the ORC complex. It has been shown that this protein and ORC1 are loosely associated with the core complex consisting of ORC2, -3, -4 and -5. Gene silencing studies with small interfering RNA demonstrated that this protein plays an essential role in coordinating chromosome replication and segregation with cytokinesis.^[4]

Interactions

ORC6 has been shown to interact with MCM5,^[5] ORC2,^[5][6] Replication protein A1,^[5] ORC4,^[5] DBF4,^[5] ORC3,^[5][6] CDC45-related protein,^[5] MCM4^[5] and Cell division cycle 7-related protein kinase.^[5]

References

1. "Human PubMed Reference:".
2. "Mouse PubMed Reference:".
3. Dhar SK, Dutta A (Nov 2000). "Identification and characterization of the human ORC6 homolog". J Biol Chem. 275 (45): 34983–8. doi:10.1074/jbc.M006069200. PMID 10945994.
4. "Entrez Gene: ORC6L origin recognition complex, subunit 6 like (yeast)".
5. Kneissl, Margot; Pütter Vera; Szalay Aladar A; Grummt Friedrich (Mar 2003). "Interaction and assembly of murine pre-replicative complex proteins in yeast and mouse cells". J. Mol. Biol. England. 327 (1): 111–28. doi:10.1016/S0022-2836(03)00079-2. ISSN 0022-2836. PMID 12614612.
6. Vashee, S; Simancek P; Challberg M D; Kelly T J (Jul 2001). "Assembly of the human origin recognition complex". J. Biol. Chem. United States. 276 (28): 26666–73. doi:10.1074/jbc.M102493200. ISSN 0021-9258. PMID 11323433.

Further reading

• Li JJ, Herskowitz I (1994). "Isolation of ORC6, a component of the yeast origin recognition complex by a one-hybrid system". Science. 262 (5141): 1870–4. doi:10.1126/science.8266075. PMID 8266075.
• Jiang W, McDonald D, Hope TJ, Hunter T (1999). "Mammalian Cdc7-Dbf4 protein kinase complex is essential for initiation of DNA replication". EMBO J. 18 (20): 5703–13. doi:10.1093/emboj/18.20.5703. PMC 1171637. PMID 10523313.
• Hartley JL, Temple GF, Brasch MA (2001). "DNA cloning using in vitro site-specific recombination". Genome Res. 10 (11): 1788–95. doi:10.1101/gr.143000. PMC 310948. PMID 11076863.
• Vashee S, Simancek P, Challberg MD, Kelly TJ (2001). "Assembly of the human origin recognition complex". J. Biol. Chem. 276 (28): 26666–73. doi:10.1074/jbc.M102493200. PMID 11323433.
• Chesnokov I, Remus D, Botchan M (2001). "Functional analysis of mutant and wild-type Drosophila origin recognition complex". Proc. Natl. Acad. Sci. U.S.A. 98 (21): 11997–2002. doi:10.1073/pnas.211342798. PMC 59756. PMID 11593009.
• Prasanth SG, Prasanth KV, Stillman B (2002). "Orc6 involved in DNA replication, chromosome segregation, and cytokinesis". Science. 297 (5583): 1026–31. doi:10.1126/science.1072802. PMID 12169736.
• Strausberg RL, Feingold EA, Grouse LH, et al. (2003). "Generation and initial analysis of more than 15,000 full-length human and mouse cDNA sequences". Proc. Natl. Acad. Sci. U.S.A. 99 (26): 16899–903. doi:10.1073/pnas.242603899. PMC 139241. PMID 12477932.
• Kneissl M, Pütter V, Szalay AA, Grummt F (2003). "Interaction and assembly of murine pre-replicative complex proteins in yeast and mouse cells". J. Mol. Biol. 327 (1): 111–28. doi:10.1016/S0022-2836(03)00079-2. PMID 12614612.
• Ota T, Suzuki Y, Nishikawa T, et al. (2004). "Complete sequencing and characterization of 21,243 full-length human cDNAs". Nat. Genet. 36 (1): 40–5. doi:10.1038/ng1285. PMID 14702039.
• Ramachandran N, Hainsworth E, Bhullar B, et al. (2004). "Self-assembling protein microarrays". Science. 305 (5680): 86–90. doi:10.1126/science.1097639. PMID 15232106.
• Gerhard DS, Wagner L, Feingold EA, et al. (2004). "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)". Genome Res. 14 (10B): 2121–7. doi:10.1101/gr.2596504. PMC 528928. PMID 15489334.
• Wiemann S, Arlt D, Huber W, et al. (2004). "From ORFeome to biology: a functional genomics pipeline". Genome Res. 14 (10B): 2136–44. doi:10.1101/gr.2576704. PMC 528930. PMID 15489336.
• Sibani S, Price GB, Zannis-Hadjopoulos M (2005). "Ku80 binds to human replication origins prior to the assembly of the ORC complex". Biochemistry. 44 (21): 7885–96. doi:10.1021/bi047327n. PMID 15910003.
• Mehrle A, Rosenfelder H, Schupp I, et al. (2006). "The LIFEdb database in 2006". Nucleic Acids Res. 34 (Database issue): D415–8. doi:10.1093/nar/gkj139. PMC 1347501. PMID 16381901.
• Olsen JV, Blagoev B, Gnad F, et al. (2006). "Global, in vivo, and site-specific phosphorylation dynamics in signaling networks". Cell. 127 (3): 635–48. doi:10.1016/j.cell.2006.09.026. PMID 17081983.