SUPT16H

SUPT16H
Available structures PDB Ortholog search: PDBe RCSB List of PDB id codes 5E5B, 4Z2M, 4Z2N
Identifiers
Aliases	SUPT16H, CDC68, FACTP140, SPT16/CDC68, SPT16, SPT16 homolog, facilitates chromatin remodeling subunit
External IDs	MGI: 1890948 HomoloGene: 5207 GeneCards: SUPT16H
Gene ontology Molecular function • poly(A) RNA binding • protein binding Cellular component • nucleus • nucleoplasm • chromosome Biological process • positive regulation of DNA-templated transcription, elongation • DNA replication • transcription elongation from RNA polymerase II promoter • nucleosome disassembly • regulation of transcription, DNA-templated • transcription from RNA polymerase II promoter • DNA repair • transcription, DNA-templated • cellular response to DNA damage stimulus • regulation of signal transduction by p53 class mediator Sources:Amigo / QuickGO
RNA expression pattern
More reference expression data
Orthologs
Species	Human	Mouse
Entrez	11198	114741
Ensembl	ENSG00000092201	ENSMUSG00000035726
UniProt	Q9Y5B9	Q920B9
RefSeq (mRNA)	NM_007192	NM_033618
RefSeq (protein)	NP_009123.1	NP_291096.2
Location (UCSC)	Chr 14: 21.35 – 21.38 Mb	Chr 14: 52.16 – 52.2 Mb
PubMed search	[1]	[2]
Wikidata

View/Edit Human

View/Edit Mouse

FACT complex subunit SPT16 is a protein that in humans is encoded by the SUPT16H gene.^[3][4][5]

Function

Transcription of protein-coding genes can be reconstituted on naked DNA with only the general transcription factors and RNA polymerase II. However, this minimal system cannot transcribe DNA packaged into chromatin, indicating that accessory factors may facilitate access to DNA. One such factor, FACT (facilitates chromatin transcription), interacts specifically with histones H2A/H2B to effect nucleosome disassembly and transcription elongation. FACT is composed of an 80 kDa subunit and a 140 kDa subunit, the latter of which is the protein encoded by this gene.^[5]

Interactions

SUPT16H has been shown to interact with BAZ1B.^[6]

References

1. "Human PubMed Reference:".
2. "Mouse PubMed Reference:".
3. Orphanides G, LeRoy G, Chang CH, Luse DS, Reinberg D (Mar 1998). "FACT, a factor that facilitates transcript elongation through nucleosomes". Cell. 92 (1): 105–16. doi:10.1016/S0092-8674(00)80903-4. PMID 9489704.
4. Keller DM, Zeng X, Wang Y, Zhang QH, Kapoor M, Shu H, Goodman R, Lozano G, Zhao Y, Lu H (Mar 2001). "A DNA damage-induced p53 serine 392 kinase complex contains CK2, hSpt16, and SSRP1". Mol Cell. 7 (2): 283–92. doi:10.1016/S1097-2765(01)00176-9. PMID 11239457.
5. "Entrez Gene: SUPT16H suppressor of Ty 16 homolog (S. cerevisiae)".
6. Kitagawa H, Fujiki R, Yoshimura K, Mezaki Y, Uematsu Y, Matsui D, Ogawa S, Unno K, Okubo M, Tokita A, Nakagawa T, Ito T, Ishimi Y, Nagasawa H, Matsumoto T, Yanagisawa J, Kato S (Jun 2003). "The chromatin-remodeling complex WINAC targets a nuclear receptor to promoters and is impaired in Williams syndrome". Cell. 113 (7): 905–17. doi:10.1016/S0092-8674(03)00436-7. PMID 12837248.

Further reading

• LeRoy G, Orphanides G, Lane WS, Reinberg D (1998). "Requirement of RSF and FACT for transcription of chromatin templates in vitro". Science. 282 (5395): 1900–4. doi:10.1126/science.282.5395.1900. PMID 9836642.
• Orphanides G, Wu WH, Lane WS, Hampsey M, Reinberg D (1999). "The chromatin-specific transcription elongation factor FACT comprises human SPT16 and SSRP1 proteins". Nature. 400 (6741): 284–8. doi:10.1038/22350. PMID 10421373.
• Kang SW, Kuzuhara T, Horikoshi M (2000). "Functional interaction of general transcription initiation factor TFIIE with general chromatin factor SPT16/CDC68". Genes Cells. 5 (4): 251–63. doi:10.1046/j.1365-2443.2000.00323.x. PMID 10792464.
• Wada T, Orphanides G, Hasegawa J, Kim DK, Shima D, Yamaguchi Y, Fukuda A, Hisatake K, Oh S, Reinberg D, Handa H (2000). "FACT relieves DSIF/NELF-mediated inhibition of transcriptional elongation and reveals functional differences between P-TEFb and TFIIH". Mol. Cell. 5 (6): 1067–72. doi:10.1016/S1097-2765(00)80272-5. PMID 10912001.
• Keller DM, Lu H (2003). "p53 serine 392 phosphorylation increases after UV through induction of the assembly of the CK2.hSPT16.SSRP1 complex". J. Biol. Chem. 277 (51): 50206–13. doi:10.1074/jbc.M209820200. PMID 12393879.
• Kitagawa H, Fujiki R, Yoshimura K, Mezaki Y, Uematsu Y, Matsui D, Ogawa S, Unno K, Okubo M, Tokita A, Nakagawa T, Ito T, Ishimi Y, Nagasawa H, Matsumoto T, Yanagisawa J, Kato S (2003). "The chromatin-remodeling complex WINAC targets a nuclear receptor to promoters and is impaired in Williams syndrome". Cell. 113 (7): 905–17. doi:10.1016/S0092-8674(03)00436-7. PMID 12837248.
• Belotserkovskaya R, Oh S, Bondarenko VA, Orphanides G, Studitsky VM, Reinberg D (2003). "FACT facilitates transcription-dependent nucleosome alteration". Science. 301 (5636): 1090–3. doi:10.1126/science.1085703. PMID 12934006.
• Li J, Hawkins IC, Harvey CD, Jennings JL, Link AJ, Patton JG (2003). "Regulation of Alternative Splicing by SRrp86 and Its Interacting Proteins". Mol. Cell. Biol. 23 (21): 7437–47. doi:10.1128/MCB.23.21.7437-7447.2003. PMC 207616. PMID 14559993.
• Tan BC, Lee SC (2004). "Nek9, a novel FACT-associated protein, modulates interphase progression". J. Biol. Chem. 279 (10): 9321–30. doi:10.1074/jbc.M311477200. PMID 14660563.
• Fryer CJ, White JB, Jones KA (2005). "Mastermind recruits CycC:CDK8 to phosphorylate the Notch ICD and coordinate activation with turnover". Mol. Cell. 16 (4): 509–20. doi:10.1016/j.molcel.2004.10.014. PMID 15546612.
• Kouskouti A, Talianidis I (2005). "Histone modifications defining active genes persist after transcriptional and mitotic inactivation". EMBO J. 24 (2): 347–57. doi:10.1038/sj.emboj.7600516. PMC 545808. PMID 15616580.
• Huang JY, Chen WH, Chang YL, Wang HT, Chuang WT, Lee SC (2006). "Modulation of nucleosome-binding activity of FACT by poly(ADP-ribosyl)ation". Nucleic Acids Res. 34 (8): 2398–407. doi:10.1093/nar/gkl241. PMC 1458519. PMID 16682447.
• Pavri R, Zhu B, Li G, Trojer P, Mandal S, Shilatifard A, Reinberg D (2006). "Histone H2B monoubiquitination functions cooperatively with FACT to regulate elongation by RNA polymerase II". Cell. 125 (4): 703–17. doi:10.1016/j.cell.2006.04.029. PMID 16713563.
• Tan BC, Chien CT, Hirose S, Lee SC (2006). "Functional cooperation between FACT and MCM helicase facilitates initiation of chromatin DNA replication". EMBO J. 25 (17): 3975–85. doi:10.1038/sj.emboj.7601271. PMC 1560368. PMID 16902406.
• Biswas D, Dutta-Biswas R, Mitra D, Shibata Y, Strahl BD, Formosa T, Stillman DJ (2006). "Opposing roles for Set2 and yFACT in regulating TBP binding at promoters". EMBO J. 25 (19): 4479–89. doi:10.1038/sj.emboj.7601333. PMC 1589996. PMID 16977311.