TBST

In molecular biology, TBST (or TTBS) is mixture of tris-buffered saline (TBS) and Polysorbate 20 (also known as Tween 20). It is a buffer used for washing nitrocellulose membrane in western blotting and microtiter plate wells in ELISA assays.

Contents of TBS-Tween

The following is a sample recipe for TBST:

• 50 mM Tris
• 150 mM NaCl
• 0.05% Tween 20

Adjust pH with HCl to pH 7.6

The simplest way to prepare a TBS-Tween solution is to use TBS-T tablets. They are formulated to give a ready to use TBST solution upon dissolution in 500 ml of deionized water.^[1]

References

1. Medicago AB, (2010) Tris buffered saline with Tween pH 7.6 specification sheet