FAD-dependent urate hydroxylase
| FAD-dependent urate hydroxylase | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Identifiers | |||||||||
| EC number | 1.14.13.113 | ||||||||
| Databases | |||||||||
| IntEnz | IntEnz view | ||||||||
| BRENDA | BRENDA entry | ||||||||
| ExPASy | NiceZyme view | ||||||||
| KEGG | KEGG entry | ||||||||
| MetaCyc | metabolic pathway | ||||||||
| PRIAM | profile | ||||||||
| PDB structures | RCSB PDB PDBe PDBsum | ||||||||
| |||||||||
FAD-dependent urate hydroxylase (EC 1.14.13.113, HpxO enzyme, FAD-dependent urate oxidase, urate hydroxylase) is an enzyme with systematic name urate,NADH:oxygen oxidoreductase (5-hydroxyisourate forming).[1] [2] A non-homologous isofunctional enzyme (NISE) to HpxO was found, and named HpyO.[3] HpyO was determined to be a typical Michaelian enzyme. These FAD-dependent urate hydroxylases are flavoproteins.
This enzyme catalyses the following chemical reaction
- urate + NADH + H+ + O2 5-hydroxyisourate + NAD+ + H2O
References
- ↑ O'Leary, S.E.; Hicks, K.A.; Ealick, S.E.; Begley, T.P. (2009). "Biochemical characterization of the HpxO enzyme from Klebsiella pneumoniae, a novel FAD-dependent urate oxidase". Biochemistry. 48 (14): 3033–3035. doi:10.1021/bi900160b. PMC 2842088
. PMID 19260710. - ↑ de la Riva L; Badia J; Aguilar J; Bender RA; Baldoma L. (2008). "The hpx genetic system for hypoxanthine assimilation as a nitrogen source in Klebsiella pneumoniae: gene organization and transcriptional regulation". Journal of Bacteriology. 190 (24): 7892–7903. doi:10.1128/JB.01022-08. PMC 2593211. PMID 18849434.
- ↑ Michiel M, Perchat N, Perret A, Tricot S, Papeil A, Besnard M, de Berardinis V, Salanoubat M, Fischer C (2012). "Microbial urate catabolism: characterization of HpyO, a non-homologous isofunctional isoform of the flavoprotein urate hydroxylase HpxO". Environmental Microbiology reports. 4 (6): 642–647. doi:10.1111/j.1758-2229.2012.00390.x. PMID 23760935.
External links
- FAD-dependent urate hydroxylase at the US National Library of Medicine Medical Subject Headings (MeSH)
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