Rajiv Krishna Saxena
Prof. Rajiv K. Saxena is an Indian immunologist. He has published research articles in scientific journals including Nature, PLOS ONE, and Nanotoxicology,[1] and is best known for developing the double in-vivo biotinylation ( DIB) technique, to study the turnover kinetics of erythrocytes.[2]
DIB involves an initial biotinylation of all erythrocytes in circulation, followed by a second biotinylation a few days after, at a lower density, that labels the biotin-negative erythrocytes that have entered since the first biotinylation. The proportions of biotin (high, low and negative) erythrocytes are enumerated by flow cytometry. The DIB technique allows tracking age-related changes on erythrocyte cohorts (Protocol A), and to simultaneously identify very young and older erythrocyte populations in the blood (Protocol B). Using this technique, investigators can reexamine: i) the relationship between age and buoyant density of erythrocytes, ii) erythrocyte destruction through a random removal mechanism, and iii) the expression of phosphatidylserine on aging erythrocytes.
References
- ↑ "Publications". rajivsaxena.info.
- ↑ "A Double in vivo Biotinylation Technique for Objective Assessment of Aging and Clearance of Mouse Erythrocytes in Blood Circulation". Transfusion Medicine and Hemotherapy. doi:10.1159/000342524.