Subcommissural organ

Subcommissural organ

Mesal aspect of a brain sectioned in the median sagittal plane. (Subcommissural organ not labeled, but region is visible, near the pineal gland.)
Details
Identifiers
Latin organum subcommissurale
MeSH Subcommissural+organ
NeuroNames hier-474
NeuroLex ID Subcommissural organ
TA A14.1.08.511
FMA 72414

Anatomical terms of neuroanatomy

The subcommissural organ (SCO) is one of the circumventricular organs. It is a small glandular structure formed by ependymal cells and hypendymal cells[1] and is located in the dorsocaudal region of the third ventricle, at the entrance of the cerebral aqueduct. The SCO is one of the first differentiated brain structures to develop.[1] It has its name because of the localization in the brain. The organ's main functions are unknown; nevertheless, some evidences suggest that the organ may participate in different process, such as the clearance of certain compounds and the circulation of the cerebrospinal fluid, and it could also play a role in some morphogenetic mechanisms.[1][2]

Structure

The subcommissural cells, specialized in the secretion of glycoproteins (including ependymin) are arranged into two polarized layers: ependyma and hypendyma. The first one (formed by a very high cylindrical cells) release their secretions into the ventricular cerebrospinal fluid and the hypendyma cells (located under the ependyma), characterized by numerous blood capillaries and glial cells, projects into the local blood vessels and to the subarachnoidal space.[1][2]

The ependymal cell bodies present a clear zonation, specially marked in certain species: the perinuclear region - the most distinct ultrastructural feature off virtually all species is the presence of large and dilated cisternae of the rough endoplasmic reticulum (RER); intermediate region – constituted mainly by the RER and the Golgi apparatus (it is not certain the involvement of the Golgi apparatus in the secretary process of the subcommissural organ); subapical region – short region, with microtubules, mitochondria and smooth endoplasmic reticulum; and an apical region – projecting a large protrusion into the ventricle.[1][2]

Function

The ependymal cells of the SCO, are also involved in the production of brain transthyretin, which is a protein involved in the transport of thyroid hormones in blood and also plays a role in transporting the cerebrospinal fluid.[3] Ependymal cells also secrete high molecular mass glycoproteins into the cerebrospinal fluid in which the bulk of them condense to form a filamentous structure, named Reissner’s fiber.[4]

The subcommissural organ/Reissner’s fiber complex is one of the factors involved in the cerebrospinal fluid reabsorption and circulation, which under normal physiological conditions, is secreted continuously, although this secretion undergoes circadian variations. Together, this complex is associated to functions regarding electrolyte and water balance.[4][5]

One of the proteins, secreted by the subcommissural organ and expressed in the central nervous system, present in the Reissner’s fiber is spondin. Subcommissural organ - spondin is a “giant” (5000 amino acids) glycoprotein (thrombospondin superfamily) found in Vertebrata. This glycoprotein shares molecular domains with axonal pathfinding molecules.[5]

The ependymal cells and the SCO–spondin secretion are suspected to play a role in homeostasis.[6]

Some studies indicate the presence of both tyrosine-hydroxylase-immunoreactive nerve fibers and dopamine receptors in the SCO ependyma.[7]

All brain capillaries of the blood-brain barrier structures have glucose transporters (GLUT1). These transporters are generally absent in leaky barrier structures. The circumventricular organs that are known to have leaky barrier capillaries were stained by fibronectin antibodies but not by GLUT1 antibodies. So, the subcommissural organ appeared to be unique showing neither GLUT1 nor capillary.[8]

The ependymal cells and the subcommissural organ–spondin secretion are suspected to play a role in homeostasis.[6] Ependymal cells also secrete high molecular mass glycoproteins into the cerebrospinal fluid in which the bulk of them condense to form a filamentous structure, named Reissner’s fiber.[4][9]

There is also evidence suggesting that the SCO activity in adult animals may be regulated by serotonin.[10]

Reissner's fiber

Main article: Reissner's fiber

The Reissner's fiber is also important on the morphogenetic neuronal processes, being involved on neuronal survival, aggregation and neurite extension. In vitro studies demonstrated that the presence of RF, in conjunction with glial cells, is essential to the survival of neuronal cells. The studies seem to point that the RF might bind some of the growth factors produced by glial cells and transport them to the neurons. On the process of neuronal aggregation, RF seems to serve as a control factor in direct cell-to-cell communication, favoring neuronal aggregation when the density of neurons is low and preventing this aggregation when the density gets higher. Although the mechanism behind this is not well understood, it is known to be linked to the different domains in SCO-spondin that are related to coagulation factors and TSRs, as referred above. Furthermore, the RF as a part on the neurite extension, promoting neurite outgrowth from both spinal and cortical neurons, in cell cultures, which may also be connected to the TSR domains of SCO-spondin.[11]

SCO-spondin, a glycoprotein of the SCO/RF complex

The primary structure of the major constituent of bovine RF, SCO-spondin, has been fully established as a large N-glycosylated protein (450 kDa).[12][11] Many lines of evidence denote that SCO-spondin plays a role in CNS development.[13] This molecule belongs to a protein superfamily exhibiting conserved motifs of the thrombospondin type 1 repeat. Proteins of this family are strongly expressed during mammalian CNS development, being involved in mechanisms of cellular adhesion and axonal pathfinding (a process by which neurons send out axons to reach the correct targets during neural development).[13]

Numerous investigations have been directed towards the identification and characterization of the secretory compounds of the SCO, clarifying partially its function. Immunoblot analyses of bovine SCO using antibodies against RF glycoproteins allowed the identification of high molecular weight glycoproteins of 540, 450, 320 and 190 kDa.[13] The 540 and the 320 kDa compounds would correspond to precursor forms.[14]

Multidomain organization

The main SCO-spondin isoform consists of multiple domains. This multidomain organization is a special feature of the Chordate Phylum, and there is a high degree of conservation in the amino acids composition in mammals.[15] The complete sequence and modular organization of SCO-spondin was first characterized in Bos taurus.[12] The structure of this protein is unique as it presents a mosaic arrangement of these domains along the backbone.

The putative function of SCO-spondin in neuronal differentiation is discussed regarding these features and homologies with other developmental molecules of the central nervous system exhibiting TSR domains, and involved in axonal guidance.[11] Peptides corresponding to SCO-spondin TSR domains strongly increased adhesivity and neuritic outgrouth of cortical neurons and induced disaggregation of spinal cord neurons. Therefore, it is a candidate to interfere with neuronal development and/or axonal guidance during ontogenesis of the central nervous system in the modulation of side-to-side and side-to-substratum interactions, and also in promoting neurite outgrowth.[11]

The identification of conserved domains including Emilin (EMI), von Willebrand factor D (vWD) low-density lipoprotein receptor type A repeats (LDLrA) domains, SCO repeats (SCORs), 26 thrombospondin type 1 repeats (TSRs), a coagulation factor 5/8 type C (FA5-8C) or discoidin motif and a C-terminal cystin knot (CTCK) domain provides a wider insight into the putative function of this protein. Similar types of arrangement was encountered in zonadhesins and immunoglobulin G (IgG) FC binding fragment which may account for SCO-spondin functional aspect on promoting cell-to-substratum adhesivity.[15]

The presence of low-density lipoprotein receptor type A (LDLrA) domains repeated ten times in the consensus sequence could provide a hint as to the function of SCORs, since LDLrA are known to interact with proteases or protease inhibitors.[16] There may be a functional link between LDLrAs and SCORs, which could both be involved in the regulation of either protease activation or protease inhibition.[15] The motifs coagulation factor 5/8 type C or discoidin and thrombospondin type 1 repeat (TSR) present in SCO-spondin consensus were initially described in blood proteins, where they were shown to play a role in coagulation or platelet aggregation. SCO-spondin and F-spondin share a similar pattern of expression in the floor plate, flexural organ and subcommissural organ and could have a redundant activity. The biological function of F-spondin and SCO-spondin on the deflection of commissural axons in the neural tube was assessed respectivelly by experiments of gain and loss of function[17] and by analyses of mutants with defective floor plate. F-spondin and SCO-spondin were both shown to promote neurite outgrowth of various neuronal cell populations, in cell culture.[18]

SCO-spondin may interfere with several biological events during early ontogenetical development of the CNS. Nevertheless, SCO-spondin is also present during the adult life, and similarly to thrombospondins, which act on various biological systems, i.e., neuronal differentiation, angiogenesis and platelet aggregation.[19]

Development

SCO

Despite being a much conserved structure throughout evolution, there are some differences on the SCO from different mammals. It is the first secretory structure to differentiate and remains fully developed and functional during the life of almost every vertebrate, excluding bats, anthropoid apes and humans. More specifically, in humans, the SCO development has a regressive nature. It reaches its apex development in fetus from 3 to 5 month old, functioning as a fully active secretory structure of the brain during this time span, and extending from the pineal recess over the posterior commissure to the mesocoelic recess. It is composed by a characteristic high columnar epithelium, which is not found in the adult SCO. Following this maxed developed state, the SCO starts regressing and in children from 3 to 4 years old it already has a vestigial character, being reduced to islet like structures on the adult. Although the remaining cells can possess some secretory material the SCO is truly vestigial in both structure and secretory function, in adults.[20]

SCO-spondin

As part of the embryonic cerebrospinal fluid (eCSF), SCO-spondin is of the uttermost importance in the development of the neuronal system, being a key protein in the balancing of differentiation and proliferation of the neuroepithelium. It starts being secreted by the diencephalic floor plate in the first embryonic stages playing an important part in the development and differentiation of structures such as the pineal gland.[21] In particular, the SCO-spondin appears to have a major role on the growth of the posterior commissure (PC), which was proved when mutants lacking SCO, and hence had no SCO-spondin, where unable to form a functional PC. On early stages of development the axonal growth is stimulated, being inhibited afterwards.[22] A steep gradient of spondin expression in the neuroepithelium signals the need for different processes to take place, favoring the fasciculation on the cephalic region and the incorporation of new neurons on the caudal region. As such, the lower concentrations of SCO-spondin in the caudal region favor the axonal outgrowth and incorporation of new axons on the posterior commissure and the higher concentrations in the cephalic region promotes the interactions between the neighboring axons.[21] In conjugation with the secretion of SCO-spondin, the midline positioning of the SCO assumes a great importance on the axon guidance process. This positioning facilitates the signaling of the turning points for the axons, through the spreading of spondin.[22] In addition to the functions in axon guidance and related growth of the posterior commissure, the SCO-spondin also appears to have a role on the adhesion of the trophoblast to the uterine walls. There is a slightly different SCO-spondin produced in the trophoblast, most likely due to alternative splicing. This spondin may recognize the classic protein on the uterine wall, facilitating the adhesion.[23]

Clinical significance

Hydrocephalus

Given that the subcommissural organ is not highly permeable and does not possess fenestrated capillaries like other subventricular organs, it has emerged as a major site of congenital hydrocephalus.[24][25] It is suggested that this is related to immunological blockage of SCO secretions and Sylvian's aqueduct malformation[26] and obliteration or turbulent cerebrospinal fluid flow due to the absence of Reissner's fibers.[13] There is evidence that in transgenic mice the overexpression of Sox3 in the dorsal midline of the diencephalon in a dose-dependent manner[27] and that the conditional inactivation of presenilin-1[28] or the lack of huntingtin[29] in wnt cell lineages leads to congenital hydrocephalus, which highlights the role of these proteins mediating the relation between the SCO and the condition. A more recent study using HTx rats reinforced the idea that the abnormal and dysfunction of the SCO precedes the development of the hydrocephalus.[4]

Other disease

It is reported that in spontaneously hypertensive rats there is a relation between SCO and hypertension due to changing in its secretor activity and protein composition.[30][31]

History

In 1860, Ernst Reissner, anatomist at the University of Dorpat, published a monograph on the microscopic structure of the spinal cord of Petromyzon fluviatilis. He described a string of 1.5 μm in diameter characterized by its high refringence, its extremely regular shape, and its lying free within the central canal. In 1866, Karl Kutschin confirmed Reissner’s observations and named the fibrous structure Reissner’s fiber.[1][2]

Edinger (1892) described, in sharks, what later was known as “the subcommissural organ”. Studnicka (1900), called attention to uncommonly tall ependymal cells covering the posterior comissure of P. fluviatilis. Sargent, also in 1900, establishes the basis of what is presently regarded as the subcomissural organ – Reissner fiber complex. Finally, in 1910, Dendy and Nicholls introduce the term “subcommissural organ” to describe this brain gland.[1][2]

The rich vascularization of the SCO was first reported by Pesonen (1940). In 1959, Helmut Hofer postulated that this organ, despite its structural and functional differences, is a highly secretory component of the circumventricular system.[1][2]

References

  1. 1 2 3 4 5 6 7 8 Rodríguez EM, Rodríguez S, Hein S (April 1998). "The subcommissural organ". Microscopy Research and Technique. 41 (2): 98–123. doi:10.1002/(SICI)1097-0029(19980415)41:2<98::AID-JEMT2>3.0.CO;2-M. PMID 9579598.
  2. 1 2 3 4 5 6 Oksche A, Rodríguez EM, Llebrez PF (1993). The Subcommissural Organ: An Ependymal Brain Gland. Berlin: Springer Verlag. ISBN 978-3-540-56336-5. OCLC 27681500.
  3. ↑ Montecinos, HA; Richter, H; Caprile, T; Rodriguez, EM (June 2005). "Synthesis of transthyretin by the ependymal cells of the subcommissural organ". Cell and Tissue Research. 320 (3): 487–499. doi:10.1007/s00441-004-0997-0. PMID 15846516.
  4. 1 2 3 4 Ortloff, A.R.; Vío, K.; Guerra, M.; Jaramillo, K.; Kaehne, T.; Jones, H.; McAllister, J.P., II; Rodríguez, E. (June 2013). "Role of the subcommissural organ in the pathogenesis of congenital hydrocephalus in the HTx rat". Cell and Tissue Research. 352 (3): 707–725. doi:10.1007/s00441-013-1615-9. PMID 23640132.
  5. 1 2 Pérez-Fígares JM, Jimenez AJ, Rodríguez EM (March 2001). "Subcommissural organ, cerebrospinal fluid circulation, and hydrocephalus". Microscopy Research and Technique. 52 (5): 591–607. doi:10.1002/1097-0029(20010301)52:5<591::AID-JEMT1043>3.0.CO;2-7. PMID 11241868.
  6. 1 2 Meiniel A (2007). "The secretory ependymal cells of the subcommissural organ: which role in hydrocephalus?". The International Journal of Biochemistry & Cell Biology. 39 (3): 463–8. doi:10.1016/j.biocel.2006.10.021. PMID 17150405.
  7. ↑ Tomé M, Jiménez AJ, Richter H, et al. (July 2004). "The subcommissural organ expresses D2, D3, D4, and D5 dopamine receptors". Cell and Tissue Research. 317 (1): 65–77. doi:10.1007/s00441-004-0900-z. PMID 15197646.
  8. ↑ Rahner-Welsch S, Vogel J, Kuschinsky W (July 1995). "Regional congruence and divergence of glucose transporters (GLUT1) and capillaries in rat brains". Journal of Cerebral Blood Flow and Metabolism. 15 (4): 681–6. doi:10.1038/jcbfm.1995.85. PMID 7790418.
  9. ↑ Ortloff AR, Vío K, Guerra M, et al. (June 2013). "Role of the subcommissural organ in the pathogenesis of congenital hydrocephalus in the HTx rat". Cell and Tissue Research. 352 (3): 707–25. doi:10.1007/s00441-013-1615-9. PMID 23640132.
  10. ↑ Richter HG, Tomé MM, Yulis CR, et al. (October 2004). "Transcription of SCO-spondin in the subcommissural organ: evidence for down-regulation mediated by serotonin". Brain Research. Molecular Brain Research. 129 (1–2): 151–62. doi:10.1016/j.molbrainres.2004.07.003. PMID 15469891.
  11. 1 2 3 4 Meiniel A (March 2001). "SCO-spondin, a glycoprotein of the subcommissural organ/Reissner's fiber complex: evidence of a potent activity on neuronal development in primary cell cultures". Microscopy Research and Technique. 52 (5): 484–95. doi:10.1002/1097-0029(20010301)52:5<484::AID-JEMT1034>3.0.CO;2-0. PMID 11241859.
  12. 1 2 Gobron S, Creveaux I, Meiniel R, et al. (November 2000). "Subcommissural organ/Reissner's fiber complex: characterization of SCO-spondin, a glycoprotein with potent activity on neurite outgrowth". Glia. 32 (2): 177–91. doi:10.1002/1098-1136(200011)32:2<177::AID-GLIA70>3.0.CO;2-V. PMID 11008217.
  13. 1 2 3 4 Vio K, Rodríguez S, Yulis CR, Oliver C, Rodríguez EM (2008). "The subcommissural organ of the rat secretes Reissner's fiber glycoproteins and CSF-soluble proteins reaching the internal and external CSF compartments". Cerebrospinal Fluid Research. 5: 3. doi:10.1186/1743-8454-5-3. PMC 2265671Freely accessible. PMID 18218138.
  14. ↑ Nualart F, Hein S, Rodríguez EM, Oksche A (October 1991). "Identification and partial characterization of the secretory glycoproteins of the bovine subcommissural organ-Reissner's fiber complex. Evidence for the existence of two precursor forms". Brain Research. Molecular Brain Research. 11 (3–4): 227–38. doi:10.1016/0169-328x(91)90031-r. PMID 1661820.
  15. 1 2 3 Meiniel O, Meiniel A (February 2007). "The complex multidomain organization of SCO-spondin protein is highly conserved in mammals". Brain Research Reviews. 53 (2): 321–7. doi:10.1016/j.brainresrev.2006.09.007. PMID 17126404.
  16. ↑ Herz J (March 2001). "The LDL receptor gene family: (un)expected signal transducers in the brain". Neuron. 29 (3): 571–81. doi:10.1016/S0896-6273(01)00234-3. PMID 11301018.
  17. ↑ Burstyn-Cohen T, Tzarfaty V, Frumkin A, Feinstein Y, Stoeckli E, Klar A (June 1999). "F-Spondin is required for accurate pathfinding of commissural axons at the floor plate". Neuron. 23 (2): 233–46. doi:10.1016/S0896-6273(00)80776-X. PMID 10399931.
  18. ↑ Meiniel A, Meiniel R, Gonçalves-Mendes N, Creveaux I, Didier R, Dastugue B (2003). "The thrombospondin type 1 repeat (TSR) and neuronal differentiation: roles of SCO-spondin oligopeptides on neuronal cell types and cell lines". International Review of Cytology. 230: 1–39. doi:10.1016/S0074-7696(03)30001-4. PMID 14692680.
  19. ↑ Adams JC, Tucker RP, Lawler J (1995). The thrombospondin gene family. Molecular Biology Intelligence Unit. ISBN 978-3-540-60399-3.
  20. ↑ Rodríguez EM, Oksche A, Montecinos H (March 2001). "Human subcommissural organ, with particular emphasis on its secretory activity during the fetal life". Microscopy Research and Technique. 52 (5): 573–90. doi:10.1002/1097-0029(20010301)52:5<573::AID-JEMT1042>3.0.CO;2-6. PMID 11241867.
  21. 1 2 Vera A, Stanic K, Montecinos H, Torrejón M, Marcellini S, Caprile T (2013). "SCO-spondin from embryonic cerebrospinal fluid is required for neurogenesis during early brain development". Frontiers in Cellular Neuroscience. 7: 80. doi:10.3389/fncel.2013.00080. PMC 3669746Freely accessible. PMID 23761733.
  22. 1 2 Hoyo-Becerra C, López-Avalos MD, Cifuentes M, Visser R, Fernández-Llebrez P, Grondona JM (February 2010). "The subcommissural organ and the development of the posterior commissure in chick embryos". Cell and Tissue Research. 339 (2): 383–95. doi:10.1007/s00441-009-0899-2. PMID 20012322.
  23. ↑ Gonçalves-Mendes N, Blanchon L, Meiniel A, Dastugue B, Sapin V (May 2004). "Placental expression of SCO-spondin during mouse and human development". Gene Expression Patterns. 4 (3): 309–14. doi:10.1016/j.modgep.2003.10.004. PMID 15053980.
  24. ↑ Huh MS, Todd MA, Picketts DJ (April 2009). "SCO-ping out the mechanisms underlying the etiology of hydrocephalus". Physiology. 24 (2): 117–26. doi:10.1152/physiol.00039.2008. PMID 19364914.
  25. ↑ McAllister JP (October 2012). "Pathophysiology of congenital and neonatal hydrocephalus". Seminars in Fetal & Neonatal Medicine. 17 (5): 285–94. doi:10.1016/j.siny.2012.06.004. PMID 22800608.
  26. ↑ Overholser MD, Whitley JR, O'Dell BL, Hogan AG (December 1954). "The ventricular system in hydrocephalic rat brains produced by a deficiency of vitamin B12 or of folic acid in the maternal diet". The Anatomical Record. 120 (4): 917–33. doi:10.1002/ar.1091200407. PMID 14350261.
  27. ↑ Lee, Kristie; Tan, Jacqueline; Morris, Michael B.; Rizzoti, Karine; Hughes, James; Cheah, Pike See; Felquer, Fernando; Liu, Xuan; Piltz, Sandra; Lovell-Badge, Robin; Thomas, Paul Q. (2012). "Congenital hydrocephalus and abnormal subcommissural organ development in Sox3 transgenic mice". PLoS ONE. 7 (1): e29041. Bibcode:2012PLoSO...729041L. doi:10.1371/journal.pone.0029041. PMC 3266892Freely accessible. PMID 22291885.
  28. ↑ Nakajima M, Matsuda K, Miyauchi N, et al. (March 2011). "Hydrocephalus and abnormal subcommissural organ in mice lacking presenilin-1 in Wnt1 cell lineages". Brain Research. 1382: 275–81. doi:10.1016/j.brainres.2011.01.048. PMC 3418702Freely accessible. PMID 21262207.
  29. ↑ Dietrich P, Shanmugasundaram R, Shuyu E, Dragatsis I (January 2009). "Congenital hydrocephalus associated with abnormal subcommissural organ in mice lacking huntingtin in Wnt1 cell lineages". Human Molecular Genetics. 18 (1): 142–50. doi:10.1093/hmg/ddn324. PMC 3298867Freely accessible. PMID 18838463.
  30. ↑ Castañeyra-Perdomo A, Carmona-Calero E, Meyer G, et al. (May 1998). "Changes in the secretory activity of the subcommissural organ of spontaneously hypertensive rats". Neuroscience Letters. 246 (3): 133–6. doi:10.1016/S0304-3940(98)00252-3. PMID 9792610.
  31. ↑ Martínez-Peña y Valenzuela I, Carmona-Calero EM, Pérez-González H, et al. (February 2006). "Alterations of the cerebrospinal fluid proteins and subcommissural organ secretion in the arterial hypertension and ventricular dilatation. A study in SHR rats". Histology and Histopathology. 21 (2): 179–85. PMID 16329042.
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