Ninein-like protein

NINL
Identifiers
Aliases	NINL, NLP, dJ691N24.1, ninein like
External IDs	MGI: 1925427 HomoloGene: 57024 GeneCards: NINL
Gene ontology Molecular function • calcium ion binding • protein binding • metal ion binding Cellular component • cytoplasm • cytosol • microtubule • microtubule organizing center • cytoskeleton Biological process • G2/M transition of mitotic cell cycle • microtubule cytoskeleton organization Sources:Amigo / QuickGO
RNA expression pattern
More reference expression data
Orthologs
Species	Human	Mouse
Entrez	22981	78177
Ensembl	ENSG00000101004	ENSMUSG00000068115
UniProt	Q9Y2I6	Q6ZQ12
RefSeq (mRNA)	NM_025176 NM_001318226	NM_207204
RefSeq (protein)	NP_079452.3 NP_001305155.1	NP_997087.2
Location (UCSC)	Chr 20: 25.45 – 25.59 Mb	Chr 2: 150.93 – 151.04 Mb
PubMed search	[1]	[2]
Wikidata

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View/Edit Mouse

Ninein-like protein is a protein that in humans is encoded by the NINL gene.^[3][4] It is part of the centrosome.^[5]

Model organisms

Ninl knockout mouse phenotype

Characteristic	Phenotype
Homozygote viability	Normal
Fertility	Normal
Body weight	Normal
Anxiety	Normal
Neurological assessment	Normal
Grip strength	Normal
Hot plate	Normal
Dysmorphology	Normal
Indirect calorimetry	Normal
Glucose tolerance test	Normal
Auditory brainstem response	Normal
DEXA	Normal
Radiography	Normal
Body temperature	Normal
Eye morphology	Normal
Clinical chemistry	Normal
Haematology	Normal
Peripheral blood lymphocytes	Normal
Micronucleus test	Normal
Heart weight	Normal
Skin Histopathology	Normal
Brain histopathology	Normal
Eye Histopathology	Normal
Salmonella infection	Normal[6]
Citrobacter infection	Normal[7]
All tests and analysis from[8][9]

Model organisms have been used in the study of NINL function. A conditional knockout mouse line, called Ninl^{tm1a(EUCOMM)Wtsi[10][11]} was generated as part of the International Knockout Mouse Consortium program — a high-throughput mutagenesis project to generate and distribute animal models of disease to interested scientists — at the Wellcome Trust Sanger Institute.^[12][13][14] Male and female animals underwent a standardized phenotypic screen to determine the effects of deletion.^[8][15] Twenty five tests were carried out on mutant mice, however no significant abnormalities were observed.^[8]

References

1. "Human PubMed Reference:".
2. "Mouse PubMed Reference:".
3. Nagase T, Ishikawa K, Suyama M, Kikuno R, Hirosawa M, Miyajima N, Tanaka A, Kotani H, Nomura N, Ohara O (Jul 1999). "Prediction of the coding sequences of unidentified human genes. XIII. The complete sequences of 100 new cDNA clones from brain which code for large proteins in vitro". DNA Res. 6 (1): 63–70. doi:10.1093/dnares/6.1.63. PMID 10231032.
4. "Entrez Gene: RP4-691N24.1 KIAA0980 protein".
5. Casenghi, Martina (2003). Functional characterization of the novel centrosomal protein Nlp (ninein-like protein) (PDF) (Ph.D thesis).
6. "Salmonella infection data for Ninl". Wellcome Trust Sanger Institute.
7. "Citrobacter infection data for Ninl". Wellcome Trust Sanger Institute.
8. Gerdin AK (2010). "The Sanger Mouse Genetics Programme: High throughput characterisation of knockout mice". Acta Ophthalmologica. 88 (S248). doi:10.1111/j.1755-3768.2010.4142.x.
9. Mouse Resources Portal, Wellcome Trust Sanger Institute.
10. "International Knockout Mouse Consortium".
11. "Mouse Genome Informatics".
12. Skarnes, W. C.; Rosen, B.; West, A. P.; Koutsourakis, M.; Bushell, W.; Iyer, V.; Mujica, A. O.; Thomas, M.; Harrow, J.; Cox, T.; Jackson, D.; Severin, J.; Biggs, P.; Fu, J.; Nefedov, M.; De Jong, P. J.; Stewart, A. F.; Bradley, A. (2011). "A conditional knockout resource for the genome-wide study of mouse gene function". Nature. 474 (7351): 337–342. doi:10.1038/nature10163. PMC 3572410. PMID 21677750.
13. Dolgin E (June 2011). "Mouse library set to be knockout". Nature. 474 (7351): 262–3. doi:10.1038/474262a. PMID 21677718.
14. Collins FS, Rossant J, Wurst W (January 2007). "A mouse for all reasons". Cell. 128 (1): 9–13. doi:10.1016/j.cell.2006.12.018. PMID 17218247.
15. van der Weyden L, White JK, Adams DJ, Logan DW (2011). "The mouse genetics toolkit: revealing function and mechanism.". Genome Biol. 12 (6): 224. doi:10.1186/gb-2011-12-6-224. PMC 3218837. PMID 21722353.

Further reading

• Wang Y, Zhan Q (2007). "Cell cycle-dependent expression of centrosomal ninein-like protein in human cells is regulated by the anaphase-promoting complex.". J. Biol. Chem. 282 (24): 17712–9. doi:10.1074/jbc.M701350200. PMID 17403670.
• Casenghi M, Barr FA, Nigg EA (2005). "Phosphorylation of Nlp by Plk1 negatively regulates its dynein-dynactin-dependent targeting to the centrosome.". J. Cell. Sci. 118 (Pt 21): 5101–8. doi:10.1242/jcs.02622. PMID 16254247.
• Rual JF, Venkatesan K, Hao T, et al. (2005). "Towards a proteome-scale map of the human protein-protein interaction network.". Nature. 437 (7062): 1173–8. doi:10.1038/nature04209. PMID 16189514.
• Gerhard DS, Wagner L, Feingold EA, et al. (2004). "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC).". Genome Res. 14 (10B): 2121–7. doi:10.1101/gr.2596504. PMC 528928. PMID 15489334.
• Ota T, Suzuki Y, Nishikawa T, et al. (2004). "Complete sequencing and characterization of 21,243 full-length human cDNAs.". Nat. Genet. 36 (1): 40–5. doi:10.1038/ng1285. PMID 14702039.
• Casenghi M, Meraldi P, Weinhart U, et al. (2003). "Polo-like kinase 1 regulates Nlp, a centrosome protein involved in microtubule nucleation.". Dev. Cell. 5 (1): 113–25. doi:10.1016/S1534-5807(03)00193-X. PMID 12852856.
• Strausberg RL, Feingold EA, Grouse LH, et al. (2003). "Generation and initial analysis of more than 15,000 full-length human and mouse cDNA sequences.". Proc. Natl. Acad. Sci. U.S.A. 99 (26): 16899–903. doi:10.1073/pnas.242603899. PMC 139241. PMID 12477932.
• Deloukas P, Matthews LH, Ashurst J, et al. (2002). "The DNA sequence and comparative analysis of human chromosome 20.". Nature. 414 (6866): 865–71. doi:10.1038/414865a. PMID 11780052.