UBE2B

UBE2B
Available structures PDB Ortholog search: PDBe RCSB List of PDB id codes 1JAS, 2Y4W, 2YB6, 2YBF
Identifiers
Aliases	UBE2B, E2-17kDa, HHR6B, HR6B, RAD6B, UBC2, ubiquitin conjugating enzyme E2 B
External IDs	MGI: 102944 HomoloGene: 101298 GeneCards: UBE2B
Gene ontology Molecular function • transferase activity • nucleotide binding • ubiquitin protein ligase activity • ubiquitin-protein transferase activity • protein binding • ATP binding • ubiquitin conjugating enzyme activity • ubiquitin protein ligase binding Cellular component • cytoplasm • membrane • plasma membrane • replication fork • HULC complex • nucleoplasm • XY body • nuclear chromatin • chromatin • nucleus Biological process • maintenance of chromatin silencing • chiasma assembly • histone H2A ubiquitination • negative regulation of cAMP-mediated signaling • postreplication repair • ubiquitin-dependent protein catabolic process • protein K63-linked ubiquitination • meiotic telomere clustering • positive regulation of reciprocal meiotic recombination • protein stabilization • protein polyubiquitination • negative regulation of apoptotic process • in utero embryonic development • protein monoubiquitination • sperm axoneme assembly • cellular response to DNA damage stimulus • protein K11-linked ubiquitination • histone lysine demethylation • protein K48-linked ubiquitination • negative regulation of histone phosphorylation • regulation of histone modification • protein ubiquitination • spermatogenesis • canonical Wnt signaling pathway • synaptonemal complex organization • response to UV • response to drug • proteasome-mediated ubiquitin-dependent protein catabolic process • protein autoubiquitination • DNA damage response, detection of DNA damage • DNA repair Sources:Amigo / QuickGO
RNA expression pattern
More reference expression data
Orthologs
Species	Human	Mouse
Entrez	7320	22210
Ensembl	ENSG00000119048	ENSMUSG00000020390
UniProt	P63146	P63147
RefSeq (mRNA)	NM_003337	NM_009458
RefSeq (protein)	NP_003328.1	NP_033484.3
Location (UCSC)	Chr 5: 134.37 – 134.39 Mb	Chr 11: 51.99 – 52 Mb
PubMed search	[1]	[2]
Wikidata

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Ubiquitin-conjugating enzyme E2 B is a protein that in humans is encoded by the UBE2B gene.^[3][4]

The modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. Ubiquitination involves at least three classes of enzymes: ubiquitin-activating enzymes, or E1s, ubiquitin-conjugating enzymes, or E2s, and ubiquitin-protein ligases, or E3s. This gene encodes a member of the E2 ubiquitin-conjugating enzyme family. This enzyme is required for post-replicative DNA damage repair. Its protein sequence is 100% identical to the mouse, rat, and rabbit homologs, which indicates that this enzyme is highly conserved in eukaryotic evolution.^[4]

Interactions

UBE2B has been shown to interact with RAD18.^[5][6]

References

1. "Human PubMed Reference:".
2. "Mouse PubMed Reference:".
3. Koken MH, Smit EM, Jaspers-Dekker I, Oostra BA, Hagemeijer A, Bootsma D, Hoeijmakers JH (May 1992). "Localization of two human homologs, HHR6A and HHR6B, of the yeast DNA repair gene RAD6 to chromosomes Xq24-q25 and 5q23-q31". Genomics. 12 (3): 447–53. doi:10.1016/0888-7543(92)90433-S. PMID 1559696.
4. "Entrez Gene: UBE2B ubiquitin-conjugating enzyme E2B (RAD6 homolog)".
5. Xin, H; Lin W; Sumanasekera W; Zhang Y; Wu X; Wang Z (July 2000). "The human RAD18 gene product interacts with HHR6A and HHR6B". Nucleic Acids Res. ENGLAND. 28 (14): 2847–54. doi:10.1093/nar/28.14.2847. PMC 102657. PMID 10908344.
6. Tateishi, S; Sakuraba Y; Masuyama S; Inoue H; Yamaizumi M (July 2000). "Dysfunction of human Rad18 results in defective postreplication repair and hypersensitivity to multiple mutagens". Proc. Natl. Acad. Sci. U.S.A. UNITED STATES. 97 (14): 7927–32. doi:10.1073/pnas.97.14.7927. ISSN 0027-8424. PMC 16647. PMID 10884424.

Further reading

• Koken MH, Reynolds P, Jaspers-Dekker I, et al. (1991). "Structural and functional conservation of two human homologs of the yeast DNA repair gene RAD6.". Proc. Natl. Acad. Sci. U.S.A. 88 (20): 8865–9. doi:10.1073/pnas.88.20.8865. PMC 52611. PMID 1717990.
• Woffendin C, Chen ZY, Staskus K, et al. (1991). "Mammalian mRNAs encoding protein closely related to ubiquitin-conjugating enzyme encoded by yeast DNA repair gene RAD6.". Biochim. Biophys. Acta. 1090 (1): 81–5. doi:10.1016/0167-4781(91)90039-o. PMID 1883845.
• Schneider R, Eckerskorn C, Lottspeich F, Schweiger M (1990). "The human ubiquitin carrier protein E2(Mr = 17,000) is homologous to the yeast DNA repair gene RAD6.". EMBO J. 9 (5): 1431–5. PMC 551831. PMID 2158443.
• Koken MH, Hoogerbrugge JW, Jasper-Dekker I, et al. (1996). "Expression of the ubiquitin-conjugating DNA repair enzymes HHR6A and B suggests a role in spermatogenesis and chromatin modification.". Dev. Biol. 173 (1): 119–32. doi:10.1006/dbio.1996.0011. PMID 8575614.
• Tateishi S, Sakuraba Y, Masuyama S, et al. (2000). "Dysfunction of human Rad18 results in defective postreplication repair and hypersensitivity to multiple mutagens.". Proc. Natl. Acad. Sci. U.S.A. 97 (14): 7927–32. doi:10.1073/pnas.97.14.7927. PMC 16647. PMID 10884424.
• Xin H, Lin W, Sumanasekera W, et al. (2000). "The human RAD18 gene product interacts with HHR6A and HHR6B.". Nucleic Acids Res. 28 (14): 2847–54. doi:10.1093/nar/28.14.2847. PMC 102657. PMID 10908344.
• Miura T, Klaus W, Ross A, et al. (2003). "The NMR structure of the class I human ubiquitin-conjugating enzyme 2b.". J. Biomol. NMR. 22 (1): 89–92. doi:10.1023/A:1013807519703. PMID 11885984.
• Shekhar MP, Lyakhovich A, Visscher DW, et al. (2002). "Rad6 overexpression induces multinucleation, centrosome amplification, abnormal mitosis, aneuploidy, and transformation.". Cancer Res. 62 (7): 2115–24. PMID 11929833.
• Strausberg RL, Feingold EA, Grouse LH, et al. (2003). "Generation and initial analysis of more than 15,000 full-length human and mouse cDNA sequences.". Proc. Natl. Acad. Sci. U.S.A. 99 (26): 16899–903. doi:10.1073/pnas.242603899. PMC 139241. PMID 12477932.
• Kwon YT, Xia Z, An JY, et al. (2003). "Female lethality and apoptosis of spermatocytes in mice lacking the UBR2 ubiquitin ligase of the N-end rule pathway.". Mol. Cell. Biol. 23 (22): 8255–71. doi:10.1128/MCB.23.22.8255-8271.2003. PMC 262401. PMID 14585983.
• Lyakhovich A, Shekhar MP (2004). "RAD6B overexpression confers chemoresistance: RAD6 expression during cell cycle and its redistribution to chromatin during DNA damage-induced response.". Oncogene. 23 (17): 3097–106. doi:10.1038/sj.onc.1207449. PMID 14981545.
• Watanabe K, Tateishi S, Kawasuji M, et al. (2005). "Rad18 guides poleta to replication stalling sites through physical interaction and PCNA monoubiquitination.". EMBO J. 23 (19): 3886–96. doi:10.1038/sj.emboj.7600383. PMC 522788. PMID 15359278.
• Gerhard DS, Wagner L, Feingold EA, et al. (2004). "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC).". Genome Res. 14 (10B): 2121–7. doi:10.1101/gr.2596504. PMC 528928. PMID 15489334.
• Stelzl U, Worm U, Lalowski M, et al. (2005). "A human protein-protein interaction network: a resource for annotating the proteome.". Cell. 122 (6): 957–68. doi:10.1016/j.cell.2005.08.029. PMID 16169070.
• Shekhar MP, Tait L, Gerard B (2007). "Essential role of T-cell factor/beta-catenin in regulation of Rad6B: a potential mechanism for Rad6B overexpression in breast cancer cells.". Mol. Cancer Res. 4 (10): 729–45. doi:10.1158/1541-7786.MCR-06-0136. PMID 17050667.